Identification and Characterization of a Newly Isolated Chitinase-Producing Strain Bacillus licheniformis SSCL-10 for Chitin Degradation
المؤلفون المشاركون
Al-Dayan, Noura
Venugopal, Divya
Dhanasekaran, Sugapriya
Sasi, Abirami
Duraipandiyan, Nagarajan
Marikani, Kannan
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-11-09
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Chitinases or chitinolytic enzymes have different applications in the field of medicine, agriculture, and industry.
The present study is aimed at developing an effective hyperchitinase-producing mutant strain of novel Bacillus licheniformis.
A simple and rapid methodology was used for screening potential chitinolytic microbiota by chemical mutagenesis with ethylmethane sulfonate and irradiation with UV.
There were 16 mutant strains exhibiting chitinase activity.
Out of the chitinase-producing strains, the strain with maximum chitinase activity was selected, the protein was partially purified by SDS-PAGE, and the strain was identified as Bacillus licheniformis (SSCL-10) with the highest specific activity of 3.4 U/mL.
The induced mutation model has been successfully implemented in the mutant EMS-13 (20.2 U/mL) that produces 5-6-fold higher yield of chitinase, whereas the mutant UV-11 (13.3 U/mL) has 3-4-fold greater chitinase activity compared to the wild strain.
The partially purified chitinase has a molecular weight of 66 kDa.
The wild strain (SSCL-10) was identified as Bacillus licheniformis using 16S rRNA sequence analysis.
This study explores the potential applications of hyperchitinase-producing bacteria in recycling and processing chitin wastes from crustaceans and shrimp, thereby adding value to the crustacean industry.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sasi, Abirami& Duraipandiyan, Nagarajan& Marikani, Kannan& Dhanasekaran, Sugapriya& Al-Dayan, Noura& Venugopal, Divya. 2020. Identification and Characterization of a Newly Isolated Chitinase-Producing Strain Bacillus licheniformis SSCL-10 for Chitin Degradation. Archaea،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1130470
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sasi, Abirami…[et al.]. Identification and Characterization of a Newly Isolated Chitinase-Producing Strain Bacillus licheniformis SSCL-10 for Chitin Degradation. Archaea No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1130470
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sasi, Abirami& Duraipandiyan, Nagarajan& Marikani, Kannan& Dhanasekaran, Sugapriya& Al-Dayan, Noura& Venugopal, Divya. Identification and Characterization of a Newly Isolated Chitinase-Producing Strain Bacillus licheniformis SSCL-10 for Chitin Degradation. Archaea. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1130470
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1130470
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر