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Identification and Characterization of a Newly Isolated Chitinase-Producing Strain Bacillus licheniformis SSCL-10 for Chitin Degradation
Joint Authors
Al-Dayan, Noura
Venugopal, Divya
Dhanasekaran, Sugapriya
Sasi, Abirami
Duraipandiyan, Nagarajan
Marikani, Kannan
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Chitinases or chitinolytic enzymes have different applications in the field of medicine, agriculture, and industry.
The present study is aimed at developing an effective hyperchitinase-producing mutant strain of novel Bacillus licheniformis.
A simple and rapid methodology was used for screening potential chitinolytic microbiota by chemical mutagenesis with ethylmethane sulfonate and irradiation with UV.
There were 16 mutant strains exhibiting chitinase activity.
Out of the chitinase-producing strains, the strain with maximum chitinase activity was selected, the protein was partially purified by SDS-PAGE, and the strain was identified as Bacillus licheniformis (SSCL-10) with the highest specific activity of 3.4 U/mL.
The induced mutation model has been successfully implemented in the mutant EMS-13 (20.2 U/mL) that produces 5-6-fold higher yield of chitinase, whereas the mutant UV-11 (13.3 U/mL) has 3-4-fold greater chitinase activity compared to the wild strain.
The partially purified chitinase has a molecular weight of 66 kDa.
The wild strain (SSCL-10) was identified as Bacillus licheniformis using 16S rRNA sequence analysis.
This study explores the potential applications of hyperchitinase-producing bacteria in recycling and processing chitin wastes from crustaceans and shrimp, thereby adding value to the crustacean industry.
American Psychological Association (APA)
Sasi, Abirami& Duraipandiyan, Nagarajan& Marikani, Kannan& Dhanasekaran, Sugapriya& Al-Dayan, Noura& Venugopal, Divya. 2020. Identification and Characterization of a Newly Isolated Chitinase-Producing Strain Bacillus licheniformis SSCL-10 for Chitin Degradation. Archaea،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1130470
Modern Language Association (MLA)
Sasi, Abirami…[et al.]. Identification and Characterization of a Newly Isolated Chitinase-Producing Strain Bacillus licheniformis SSCL-10 for Chitin Degradation. Archaea No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1130470
American Medical Association (AMA)
Sasi, Abirami& Duraipandiyan, Nagarajan& Marikani, Kannan& Dhanasekaran, Sugapriya& Al-Dayan, Noura& Venugopal, Divya. Identification and Characterization of a Newly Isolated Chitinase-Producing Strain Bacillus licheniformis SSCL-10 for Chitin Degradation. Archaea. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1130470
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130470