The Structure of ampG Gene in Pseudomonas aeruginosa and Its Effect on Drug Resistance
المؤلفون المشاركون
Bao, Qiyu
Chang, Qingli
Wu, Chongyang
Lin, Chaoqing
Li, Peizhen
Zhang, Kaibo
Xu, Lei
Liu, Yabo
Lu, Junwan
Cheng, Cong
Hu, Yunliang
Lu, Shunfei
Li, Jinsong
المصدر
Canadian Journal of Infectious Diseases and Medical Microbiology
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-11-26
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
In order to study the relationship between the structure and function of AmpG, structure, site-specific mutation, and gene complementary experiments have been performed against the clinical isolates of Pseudomonas aeruginosa.
We found that there are 51 nucleotide variations at 34 loci over the ampG genes from 24 of 35 P.
aeruginosa strains detected, of which 7 nucleotide variations resulted in amino acid change.
The ampG variants with the changed nucleotides (amino acids) could complement the function of ampG deleted PA01 (PA01ΔG).
The ampicillin minimum inhibitory concentration (MIC) of PA01ΔG complemented with 32 ampG variants was up to 512 μg/ml, similar to the original PA01 (P.
aeruginosa PA01).
Furthermore, site-directed mutation of two conservative amino acids (I53 and W90) showed that when I53 was mutated to 53S or 53T (I53S or I53T), the ampicillin MIC level dropped drastically, and the activity of AmpC β-lactamase decreased as well.
By contrast, the ampicillin MIC and the activity of AmpC β-lactamase remained unchanged for W90R and W90S mutants.
Our studies demonstrated that although nucleotide variations occurred in most of the ampG genes, the structure of AmpG protein in clinical isolates is stable, and conservative amino acid is necessary to maintain normal function of AmpG.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chang, Qingli& Wu, Chongyang& Lin, Chaoqing& Li, Peizhen& Zhang, Kaibo& Xu, Lei…[et al.]. 2018. The Structure of ampG Gene in Pseudomonas aeruginosa and Its Effect on Drug Resistance. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1131222
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chang, Qingli…[et al.]. The Structure of ampG Gene in Pseudomonas aeruginosa and Its Effect on Drug Resistance. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1131222
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chang, Qingli& Wu, Chongyang& Lin, Chaoqing& Li, Peizhen& Zhang, Kaibo& Xu, Lei…[et al.]. The Structure of ampG Gene in Pseudomonas aeruginosa and Its Effect on Drug Resistance. Canadian Journal of Infectious Diseases and Medical Microbiology. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1131222
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1131222
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر