The Structure of ampG Gene in Pseudomonas aeruginosa and Its Effect on Drug Resistance
Joint Authors
Bao, Qiyu
Chang, Qingli
Wu, Chongyang
Lin, Chaoqing
Li, Peizhen
Zhang, Kaibo
Xu, Lei
Liu, Yabo
Lu, Junwan
Cheng, Cong
Hu, Yunliang
Lu, Shunfei
Li, Jinsong
Source
Canadian Journal of Infectious Diseases and Medical Microbiology
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-26
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
In order to study the relationship between the structure and function of AmpG, structure, site-specific mutation, and gene complementary experiments have been performed against the clinical isolates of Pseudomonas aeruginosa.
We found that there are 51 nucleotide variations at 34 loci over the ampG genes from 24 of 35 P.
aeruginosa strains detected, of which 7 nucleotide variations resulted in amino acid change.
The ampG variants with the changed nucleotides (amino acids) could complement the function of ampG deleted PA01 (PA01ΔG).
The ampicillin minimum inhibitory concentration (MIC) of PA01ΔG complemented with 32 ampG variants was up to 512 μg/ml, similar to the original PA01 (P.
aeruginosa PA01).
Furthermore, site-directed mutation of two conservative amino acids (I53 and W90) showed that when I53 was mutated to 53S or 53T (I53S or I53T), the ampicillin MIC level dropped drastically, and the activity of AmpC β-lactamase decreased as well.
By contrast, the ampicillin MIC and the activity of AmpC β-lactamase remained unchanged for W90R and W90S mutants.
Our studies demonstrated that although nucleotide variations occurred in most of the ampG genes, the structure of AmpG protein in clinical isolates is stable, and conservative amino acid is necessary to maintain normal function of AmpG.
American Psychological Association (APA)
Chang, Qingli& Wu, Chongyang& Lin, Chaoqing& Li, Peizhen& Zhang, Kaibo& Xu, Lei…[et al.]. 2018. The Structure of ampG Gene in Pseudomonas aeruginosa and Its Effect on Drug Resistance. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1131222
Modern Language Association (MLA)
Chang, Qingli…[et al.]. The Structure of ampG Gene in Pseudomonas aeruginosa and Its Effect on Drug Resistance. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1131222
American Medical Association (AMA)
Chang, Qingli& Wu, Chongyang& Lin, Chaoqing& Li, Peizhen& Zhang, Kaibo& Xu, Lei…[et al.]. The Structure of ampG Gene in Pseudomonas aeruginosa and Its Effect on Drug Resistance. Canadian Journal of Infectious Diseases and Medical Microbiology. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1131222
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131222