Aseptic Technology for Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into Clean Liquid Air: Apoptosis, Necrosis, Motility, and Viability
المؤلفون المشاركون
Katkov, Igor I.
Wang, Mengying
Todorov, Plamen
Rahimi, Gohar
Isachenko, Vladimir
Mallmann, Peter
Isachenko, Evgenia
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-01-25
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
This study aimed to compare the quality of human spermatozoa vitrified by direct plunging into liquid nitrogen vs.
liquid air.
Spermatozoa were divided into three groups: fresh spermatozoa (Group F) were used as a control.
Spermatozoa suspension (20 μl) was vitrified in open granules by direct dropping into liquid nitrogen (Group LN) or clean liquid air (Group LA).
After warming at 37°C, the progressive motility rate of Group F was reduced from 65.9 ± 2.5% to 34.0 ± 1.9% (Group LN) and 38.1 ± 2.3% (Group LA), respectively (P1-2,3 < 0.05).
The reductions in viability were 65.6 ± 2.2%, 29.0 ± 1.8%, and 36.6 ± 2.6% for Groups F, LN, and LA, respectively (P1-2,3 < 0.05).
Comparing spermatozoa vitrified in liquid nitrogen vs.
liquid air, no significant differences were detected in motility (34.0 ± 1.9% vs.
38.1 ± 2.3%), viability (29.0 ± 1.8% vs.
36.6 ± 2.6%), early apoptosis (13.8 ± 1.5% vs.
14.3 ± 1.8%), late apoptosis (45.5 ± 1.8% vs.
43.7 ± 2.2%), and necrosis (19.5 ± 2.0% vs.
15.0 ± 1.8%; p>0.01 for all respective differences).
There was a statistical tendency for increasing rates of “progressive motility” and “viability” and decreasing rates of “apoptosis” and “necrosis” when comparing spermatozoa vitrified in liquid air vs.
liquid nitrogen.
It is concluded that cryoprotectant-free vitrification by the direct dropping of human spermatozoa in a clean cooling agent (liquid air) is a good alternative to the use of nonsterile liquid nitrogen and can be used to cool cells while minimising the risk of microbial contamination.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Mengying& Isachenko, Evgenia& Todorov, Plamen& Rahimi, Gohar& Mallmann, Peter& Katkov, Igor I.…[et al.]. 2020. Aseptic Technology for Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into Clean Liquid Air: Apoptosis, Necrosis, Motility, and Viability. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1132828
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Mengying…[et al.]. Aseptic Technology for Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into Clean Liquid Air: Apoptosis, Necrosis, Motility, and Viability. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1132828
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Mengying& Isachenko, Evgenia& Todorov, Plamen& Rahimi, Gohar& Mallmann, Peter& Katkov, Igor I.…[et al.]. Aseptic Technology for Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into Clean Liquid Air: Apoptosis, Necrosis, Motility, and Viability. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1132828
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1132828
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر