Aseptic Technology for Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into Clean Liquid Air: Apoptosis, Necrosis, Motility, and Viability
Joint Authors
Katkov, Igor I.
Wang, Mengying
Todorov, Plamen
Rahimi, Gohar
Isachenko, Vladimir
Mallmann, Peter
Isachenko, Evgenia
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-25
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This study aimed to compare the quality of human spermatozoa vitrified by direct plunging into liquid nitrogen vs.
liquid air.
Spermatozoa were divided into three groups: fresh spermatozoa (Group F) were used as a control.
Spermatozoa suspension (20 μl) was vitrified in open granules by direct dropping into liquid nitrogen (Group LN) or clean liquid air (Group LA).
After warming at 37°C, the progressive motility rate of Group F was reduced from 65.9 ± 2.5% to 34.0 ± 1.9% (Group LN) and 38.1 ± 2.3% (Group LA), respectively (P1-2,3 < 0.05).
The reductions in viability were 65.6 ± 2.2%, 29.0 ± 1.8%, and 36.6 ± 2.6% for Groups F, LN, and LA, respectively (P1-2,3 < 0.05).
Comparing spermatozoa vitrified in liquid nitrogen vs.
liquid air, no significant differences were detected in motility (34.0 ± 1.9% vs.
38.1 ± 2.3%), viability (29.0 ± 1.8% vs.
36.6 ± 2.6%), early apoptosis (13.8 ± 1.5% vs.
14.3 ± 1.8%), late apoptosis (45.5 ± 1.8% vs.
43.7 ± 2.2%), and necrosis (19.5 ± 2.0% vs.
15.0 ± 1.8%; p>0.01 for all respective differences).
There was a statistical tendency for increasing rates of “progressive motility” and “viability” and decreasing rates of “apoptosis” and “necrosis” when comparing spermatozoa vitrified in liquid air vs.
liquid nitrogen.
It is concluded that cryoprotectant-free vitrification by the direct dropping of human spermatozoa in a clean cooling agent (liquid air) is a good alternative to the use of nonsterile liquid nitrogen and can be used to cool cells while minimising the risk of microbial contamination.
American Psychological Association (APA)
Wang, Mengying& Isachenko, Evgenia& Todorov, Plamen& Rahimi, Gohar& Mallmann, Peter& Katkov, Igor I.…[et al.]. 2020. Aseptic Technology for Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into Clean Liquid Air: Apoptosis, Necrosis, Motility, and Viability. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1132828
Modern Language Association (MLA)
Wang, Mengying…[et al.]. Aseptic Technology for Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into Clean Liquid Air: Apoptosis, Necrosis, Motility, and Viability. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1132828
American Medical Association (AMA)
Wang, Mengying& Isachenko, Evgenia& Todorov, Plamen& Rahimi, Gohar& Mallmann, Peter& Katkov, Igor I.…[et al.]. Aseptic Technology for Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into Clean Liquid Air: Apoptosis, Necrosis, Motility, and Viability. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1132828
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1132828