Small Molecule Supplements Improve Cultured Megakaryocyte Polyploidization by Modulating Multiple Cell Cycle Regulators
المؤلفون المشاركون
Yue, Wen
Zou, Xiaojing
Qu, Mingyi
Fang, Fang
Fan, Zeng
Chen, Lin
Xie, Xiaoyan
Pei, Xuetao
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-10-19
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Platelets (PLTs) are produced by megakaryocytes (MKs) that completed differentiation and endomitosis.
Endomitosis is an important process in which the cell replicates its DNA without cytokinesis and develops highly polyploid MK.
In this study, to gain a better PLTs production, four small molecules (Rho-Rock inhibitor (RRI), nicotinamide (NIC), Src inhibitor (SI), and Aurora B inhibitor (ABI)) and their combinations were surveyed as MK culture supplements for promoting polyploidization.
Three leukemia cell lines as well as primary mononuclear cells were chosen in the function and mechanism studies of the small molecules.
In an optimal culture method, cells were treated with different small molecules and their combinations.
The impact of the small molecules on megakaryocytic surface marker expression, polyploidy, proliferation, and apoptosis was examined for the best MK polyploidization supplement.
The elaborate analysis confirmed that the combination of SI and RRI together with our MK induction system might result in efficient ploidy promotion.
Our experiments demonstrated that, besides direct downregulation on the expression of cytoskeleton protein actin, SI and RRI could significantly enhance the level of cyclins through the suppression of p53 and p21.
The verified small molecule combination might be further used in the in vitro PLT manufacture and clinical applications.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zou, Xiaojing& Qu, Mingyi& Fang, Fang& Fan, Zeng& Chen, Lin& Yue, Wen…[et al.]. 2017. Small Molecule Supplements Improve Cultured Megakaryocyte Polyploidization by Modulating Multiple Cell Cycle Regulators. BioMed Research International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1134652
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zou, Xiaojing…[et al.]. Small Molecule Supplements Improve Cultured Megakaryocyte Polyploidization by Modulating Multiple Cell Cycle Regulators. BioMed Research International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1134652
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zou, Xiaojing& Qu, Mingyi& Fang, Fang& Fan, Zeng& Chen, Lin& Yue, Wen…[et al.]. Small Molecule Supplements Improve Cultured Megakaryocyte Polyploidization by Modulating Multiple Cell Cycle Regulators. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1134652
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1134652
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر