Small Molecule Supplements Improve Cultured Megakaryocyte Polyploidization by Modulating Multiple Cell Cycle Regulators
Joint Authors
Yue, Wen
Zou, Xiaojing
Qu, Mingyi
Fang, Fang
Fan, Zeng
Chen, Lin
Xie, Xiaoyan
Pei, Xuetao
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-19
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Platelets (PLTs) are produced by megakaryocytes (MKs) that completed differentiation and endomitosis.
Endomitosis is an important process in which the cell replicates its DNA without cytokinesis and develops highly polyploid MK.
In this study, to gain a better PLTs production, four small molecules (Rho-Rock inhibitor (RRI), nicotinamide (NIC), Src inhibitor (SI), and Aurora B inhibitor (ABI)) and their combinations were surveyed as MK culture supplements for promoting polyploidization.
Three leukemia cell lines as well as primary mononuclear cells were chosen in the function and mechanism studies of the small molecules.
In an optimal culture method, cells were treated with different small molecules and their combinations.
The impact of the small molecules on megakaryocytic surface marker expression, polyploidy, proliferation, and apoptosis was examined for the best MK polyploidization supplement.
The elaborate analysis confirmed that the combination of SI and RRI together with our MK induction system might result in efficient ploidy promotion.
Our experiments demonstrated that, besides direct downregulation on the expression of cytoskeleton protein actin, SI and RRI could significantly enhance the level of cyclins through the suppression of p53 and p21.
The verified small molecule combination might be further used in the in vitro PLT manufacture and clinical applications.
American Psychological Association (APA)
Zou, Xiaojing& Qu, Mingyi& Fang, Fang& Fan, Zeng& Chen, Lin& Yue, Wen…[et al.]. 2017. Small Molecule Supplements Improve Cultured Megakaryocyte Polyploidization by Modulating Multiple Cell Cycle Regulators. BioMed Research International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1134652
Modern Language Association (MLA)
Zou, Xiaojing…[et al.]. Small Molecule Supplements Improve Cultured Megakaryocyte Polyploidization by Modulating Multiple Cell Cycle Regulators. BioMed Research International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1134652
American Medical Association (AMA)
Zou, Xiaojing& Qu, Mingyi& Fang, Fang& Fan, Zeng& Chen, Lin& Yue, Wen…[et al.]. Small Molecule Supplements Improve Cultured Megakaryocyte Polyploidization by Modulating Multiple Cell Cycle Regulators. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1134652
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134652