Effect of Selenium on Expression of Apoptosis-Related Genes in Cryomedia of Mice Ovary after Vitrification
المؤلفون المشاركون
Nori-Garavand, Reza
Hormozi, Maryam
Narimani, Leila
Beigi Boroujeni, Nasim
Rajabzadeh, Asghar
Zarei, Leila
Beigi Boroujeni, Masoud
Beigi Boroujeni, Mandana
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-09-23
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Introduction.
Freezing of ovarian tissue is used for preservation of fertility.
The freezing-thawing process is accompanied by oxidative stress and induction of apoptosis.
Apoptosis is a complex process that has been studied in animal models.
The present study was aimed at investigating the effect of selenium on suppression of apoptosis during vitrification-thawing process of mice ovary via studying expression of apoptosis-related genes, and also, we aimed to design statistical models for the roles of single genes and gene-gene interactions in suppression of apoptosis.
Methods.
A total of 10 right ovary samples from 10 mice were randomly divided into two groups of selenium treatment (at dose 5 μg/ml sodium selenite, through adding to the media) and control group.
Vitrification-thawing process was done according to the existed protocols.
Real-time PCR was used for gene expression study.
The apoptosis gene profile included P53, Bax, Fas, and Bcl-2.
General linear model was applied to study single gene associations and gene-gene interactions.
Results.
From the studied genes, P53 showed a significant downregulation in the selenium group in comparison to the control group (∆∆CT=1.96; P=0.013; relative expression RE=0.28).
Bcl-2 showed a significant upregulation in the selenium group in comparison to the control group (∆∆CT=−2.49; P<0.001; RE=3.49).
No significant result was found for other genes.
According to the multiple models, Bcl-2 showed a protective single gene association (beta=−0.33; P=0.032), and Fas∗Bcl-2 interaction was significantly positive (beta=0.19; P=0.036).
Conclusion.
Addition of selenium to cryomedia of vitrification-thawing process could reduce the apoptosis induced by freezing-thawing stress in mice ovary via downregulation of P53 and upregulation of Bcl-2 at transcription level.
Multivariable statistical models should be performed in future researches to study biological systems.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nori-Garavand, Reza& Hormozi, Maryam& Narimani, Leila& Beigi Boroujeni, Nasim& Rajabzadeh, Asghar& Zarei, Leila…[et al.]. 2020. Effect of Selenium on Expression of Apoptosis-Related Genes in Cryomedia of Mice Ovary after Vitrification. BioMed Research International،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1134734
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nori-Garavand, Reza…[et al.]. Effect of Selenium on Expression of Apoptosis-Related Genes in Cryomedia of Mice Ovary after Vitrification. BioMed Research International No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1134734
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nori-Garavand, Reza& Hormozi, Maryam& Narimani, Leila& Beigi Boroujeni, Nasim& Rajabzadeh, Asghar& Zarei, Leila…[et al.]. Effect of Selenium on Expression of Apoptosis-Related Genes in Cryomedia of Mice Ovary after Vitrification. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1134734
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1134734
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر