Effect of Selenium on Expression of Apoptosis-Related Genes in Cryomedia of Mice Ovary after Vitrification
Joint Authors
Nori-Garavand, Reza
Hormozi, Maryam
Narimani, Leila
Beigi Boroujeni, Nasim
Rajabzadeh, Asghar
Zarei, Leila
Beigi Boroujeni, Masoud
Beigi Boroujeni, Mandana
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Introduction.
Freezing of ovarian tissue is used for preservation of fertility.
The freezing-thawing process is accompanied by oxidative stress and induction of apoptosis.
Apoptosis is a complex process that has been studied in animal models.
The present study was aimed at investigating the effect of selenium on suppression of apoptosis during vitrification-thawing process of mice ovary via studying expression of apoptosis-related genes, and also, we aimed to design statistical models for the roles of single genes and gene-gene interactions in suppression of apoptosis.
Methods.
A total of 10 right ovary samples from 10 mice were randomly divided into two groups of selenium treatment (at dose 5 μg/ml sodium selenite, through adding to the media) and control group.
Vitrification-thawing process was done according to the existed protocols.
Real-time PCR was used for gene expression study.
The apoptosis gene profile included P53, Bax, Fas, and Bcl-2.
General linear model was applied to study single gene associations and gene-gene interactions.
Results.
From the studied genes, P53 showed a significant downregulation in the selenium group in comparison to the control group (∆∆CT=1.96; P=0.013; relative expression RE=0.28).
Bcl-2 showed a significant upregulation in the selenium group in comparison to the control group (∆∆CT=−2.49; P<0.001; RE=3.49).
No significant result was found for other genes.
According to the multiple models, Bcl-2 showed a protective single gene association (beta=−0.33; P=0.032), and Fas∗Bcl-2 interaction was significantly positive (beta=0.19; P=0.036).
Conclusion.
Addition of selenium to cryomedia of vitrification-thawing process could reduce the apoptosis induced by freezing-thawing stress in mice ovary via downregulation of P53 and upregulation of Bcl-2 at transcription level.
Multivariable statistical models should be performed in future researches to study biological systems.
American Psychological Association (APA)
Nori-Garavand, Reza& Hormozi, Maryam& Narimani, Leila& Beigi Boroujeni, Nasim& Rajabzadeh, Asghar& Zarei, Leila…[et al.]. 2020. Effect of Selenium on Expression of Apoptosis-Related Genes in Cryomedia of Mice Ovary after Vitrification. BioMed Research International،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1134734
Modern Language Association (MLA)
Nori-Garavand, Reza…[et al.]. Effect of Selenium on Expression of Apoptosis-Related Genes in Cryomedia of Mice Ovary after Vitrification. BioMed Research International No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1134734
American Medical Association (AMA)
Nori-Garavand, Reza& Hormozi, Maryam& Narimani, Leila& Beigi Boroujeni, Nasim& Rajabzadeh, Asghar& Zarei, Leila…[et al.]. Effect of Selenium on Expression of Apoptosis-Related Genes in Cryomedia of Mice Ovary after Vitrification. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1134734
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134734