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Enhancement of Nucleoside Production in Hirsutella sinensis Based on Biosynthetic Pathway Analysis
المؤلفون المشاركون
Xue, Ya-Ping
Zheng, Yu-Guo
Lin, Shan
Liu, Zhi-Qiang
Zhang, Bo
Baker, Peter James
Chen, Mao-Sheng
Wu, Hui
Xu, Feng
Yuan, Shui-Jin
Teng, Yi
Wu, Ling-Fang
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-11-29
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
To enhance nucleoside production in Hirsutella sinensis, the biosynthetic pathways of purine and pyrimidine nucleosides were constructed and verified.
The differential expression analysis showed that purine nucleoside phosphorylase, inosine monophosphate dehydrogenase, and guanosine monophosphate synthase genes involved in purine nucleotide biosynthesis were significantly upregulated 16.56-fold, 8-fold, and 5.43-fold, respectively.
Moreover, dihydroorotate dehydrogenase, uridine nucleosidase, uridine/cytidine monophosphate kinase, and inosine triphosphate pyrophosphatase genes participating in pyrimidine nucleoside biosynthesis were upregulated 4.53-fold, 10.63-fold, 4.26-fold, and 5.98-fold, respectively.
To enhance the nucleoside production, precursors for synthesis of nucleosides were added based on the analysis of biosynthetic pathways.
Uridine and cytidine contents, respectively, reached 5.04 mg/g and 3.54 mg/g when adding 2 mg/mL of ribose, resulting in an increase of 28.6% and 296% compared with the control, respectively.
Meanwhile, uridine and cytidine contents, respectively, reached 10.83 mg/g 2.12 mg/g when adding 0.3 mg/mL of uracil, leading to an increase of 176.3% and 137.1%, respectively.
This report indicated that fermentation regulation was an effective way to enhance the nucleoside production in H.
sinensis based on biosynthetic pathway analysis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Zhi-Qiang& Zhang, Bo& Lin, Shan& Baker, Peter James& Chen, Mao-Sheng& Xue, Ya-Ping…[et al.]. 2017. Enhancement of Nucleoside Production in Hirsutella sinensis Based on Biosynthetic Pathway Analysis. BioMed Research International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1134871
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Zhi-Qiang…[et al.]. Enhancement of Nucleoside Production in Hirsutella sinensis Based on Biosynthetic Pathway Analysis. BioMed Research International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1134871
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Zhi-Qiang& Zhang, Bo& Lin, Shan& Baker, Peter James& Chen, Mao-Sheng& Xue, Ya-Ping…[et al.]. Enhancement of Nucleoside Production in Hirsutella sinensis Based on Biosynthetic Pathway Analysis. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1134871
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1134871
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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