Enhancement of Nucleoside Production in Hirsutella sinensis Based on Biosynthetic Pathway Analysis
Joint Authors
Xue, Ya-Ping
Zheng, Yu-Guo
Lin, Shan
Liu, Zhi-Qiang
Zhang, Bo
Baker, Peter James
Chen, Mao-Sheng
Wu, Hui
Xu, Feng
Yuan, Shui-Jin
Teng, Yi
Wu, Ling-Fang
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-29
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
To enhance nucleoside production in Hirsutella sinensis, the biosynthetic pathways of purine and pyrimidine nucleosides were constructed and verified.
The differential expression analysis showed that purine nucleoside phosphorylase, inosine monophosphate dehydrogenase, and guanosine monophosphate synthase genes involved in purine nucleotide biosynthesis were significantly upregulated 16.56-fold, 8-fold, and 5.43-fold, respectively.
Moreover, dihydroorotate dehydrogenase, uridine nucleosidase, uridine/cytidine monophosphate kinase, and inosine triphosphate pyrophosphatase genes participating in pyrimidine nucleoside biosynthesis were upregulated 4.53-fold, 10.63-fold, 4.26-fold, and 5.98-fold, respectively.
To enhance the nucleoside production, precursors for synthesis of nucleosides were added based on the analysis of biosynthetic pathways.
Uridine and cytidine contents, respectively, reached 5.04 mg/g and 3.54 mg/g when adding 2 mg/mL of ribose, resulting in an increase of 28.6% and 296% compared with the control, respectively.
Meanwhile, uridine and cytidine contents, respectively, reached 10.83 mg/g 2.12 mg/g when adding 0.3 mg/mL of uracil, leading to an increase of 176.3% and 137.1%, respectively.
This report indicated that fermentation regulation was an effective way to enhance the nucleoside production in H.
sinensis based on biosynthetic pathway analysis.
American Psychological Association (APA)
Liu, Zhi-Qiang& Zhang, Bo& Lin, Shan& Baker, Peter James& Chen, Mao-Sheng& Xue, Ya-Ping…[et al.]. 2017. Enhancement of Nucleoside Production in Hirsutella sinensis Based on Biosynthetic Pathway Analysis. BioMed Research International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1134871
Modern Language Association (MLA)
Liu, Zhi-Qiang…[et al.]. Enhancement of Nucleoside Production in Hirsutella sinensis Based on Biosynthetic Pathway Analysis. BioMed Research International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1134871
American Medical Association (AMA)
Liu, Zhi-Qiang& Zhang, Bo& Lin, Shan& Baker, Peter James& Chen, Mao-Sheng& Xue, Ya-Ping…[et al.]. Enhancement of Nucleoside Production in Hirsutella sinensis Based on Biosynthetic Pathway Analysis. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1134871
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134871