In Vivo Development of Polymyxin B Resistance in Klebsiella pneumoniae owing to a 42 bp Deletion in the Sequence of phoQ
المؤلفون المشاركون
Xu, Qingqing
Xu, Teng
Zhuang, Yuan
Liu, Xiaofen
Li, Ying
Chen, Yijian
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-04-22
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Polymyxins resistance has emerged worldwide and is threatening the treatment efficacy of multidrug resistant Klebsiella pneumoniae in humans and animals.
In this research, we employed whole-genome sequencing (WGS) to investigate the polymyxin B resistance mechanism in selected polymyxin B-susceptible and polymyxin B-resistant K.
pneumoniae, isolated from one patient of Huashan Hospital affiliated to Fudan University.
The WGS results showed that the two K.
pneumoniae all belong to ST11.
The average nucleotide identity between the two K.
pneumoniae was nearly 100%.
No sense mutations of polymyxins resistance associated genes (pmrA, pmrB, phoP, mgrB) were observed in polymyxin B-resistant K.
pneumonia (PRKP) compared to the polymyxin B-susceptible isolate.
A 42 bp deletion was found in the sequence of phoQ in PRKP.
The deletion of amino acid occurred on the periplasmic domain of PhoQ protein.
We speculate that this is the domain that MgrB protein interact with the PhoQ protein and negatively regulate the PhoP/PhoQ system.
qRT-PCR analysis revealed an overexpression of the pmrA (6.8-fold), pmrB (151.9-fold), pmrC (14.5-fold), pmrK (287.9-fold), phoP (14.5-fold), and phoQ (16.8-fold) genes in the polymyxin B-resistant isolate compared to the expression of the polymyxin B-susceptible K.
pneumoniae isolate, suggesting that the phoQ deletion maybe responsible for the increased expression levels of those genes.
In conclusion, this study identified a 42 bp deletion in the sequence of phoQ as being responsible for the overexpression of pmrCAB and pmrHFIJKLM operons, leading to resistance to polymyxin B.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xu, Qingqing& Xu, Teng& Zhuang, Yuan& Liu, Xiaofen& Li, Ying& Chen, Yijian. 2020. In Vivo Development of Polymyxin B Resistance in Klebsiella pneumoniae owing to a 42 bp Deletion in the Sequence of phoQ. BioMed Research International،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1135050
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xu, Qingqing…[et al.]. In Vivo Development of Polymyxin B Resistance in Klebsiella pneumoniae owing to a 42 bp Deletion in the Sequence of phoQ. BioMed Research International No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1135050
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xu, Qingqing& Xu, Teng& Zhuang, Yuan& Liu, Xiaofen& Li, Ying& Chen, Yijian. In Vivo Development of Polymyxin B Resistance in Klebsiella pneumoniae owing to a 42 bp Deletion in the Sequence of phoQ. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1135050
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1135050
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر