In Vivo Development of Polymyxin B Resistance in Klebsiella pneumoniae owing to a 42 bp Deletion in the Sequence of phoQ
Joint Authors
Xu, Qingqing
Xu, Teng
Zhuang, Yuan
Liu, Xiaofen
Li, Ying
Chen, Yijian
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-22
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Polymyxins resistance has emerged worldwide and is threatening the treatment efficacy of multidrug resistant Klebsiella pneumoniae in humans and animals.
In this research, we employed whole-genome sequencing (WGS) to investigate the polymyxin B resistance mechanism in selected polymyxin B-susceptible and polymyxin B-resistant K.
pneumoniae, isolated from one patient of Huashan Hospital affiliated to Fudan University.
The WGS results showed that the two K.
pneumoniae all belong to ST11.
The average nucleotide identity between the two K.
pneumoniae was nearly 100%.
No sense mutations of polymyxins resistance associated genes (pmrA, pmrB, phoP, mgrB) were observed in polymyxin B-resistant K.
pneumonia (PRKP) compared to the polymyxin B-susceptible isolate.
A 42 bp deletion was found in the sequence of phoQ in PRKP.
The deletion of amino acid occurred on the periplasmic domain of PhoQ protein.
We speculate that this is the domain that MgrB protein interact with the PhoQ protein and negatively regulate the PhoP/PhoQ system.
qRT-PCR analysis revealed an overexpression of the pmrA (6.8-fold), pmrB (151.9-fold), pmrC (14.5-fold), pmrK (287.9-fold), phoP (14.5-fold), and phoQ (16.8-fold) genes in the polymyxin B-resistant isolate compared to the expression of the polymyxin B-susceptible K.
pneumoniae isolate, suggesting that the phoQ deletion maybe responsible for the increased expression levels of those genes.
In conclusion, this study identified a 42 bp deletion in the sequence of phoQ as being responsible for the overexpression of pmrCAB and pmrHFIJKLM operons, leading to resistance to polymyxin B.
American Psychological Association (APA)
Xu, Qingqing& Xu, Teng& Zhuang, Yuan& Liu, Xiaofen& Li, Ying& Chen, Yijian. 2020. In Vivo Development of Polymyxin B Resistance in Klebsiella pneumoniae owing to a 42 bp Deletion in the Sequence of phoQ. BioMed Research International،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1135050
Modern Language Association (MLA)
Xu, Qingqing…[et al.]. In Vivo Development of Polymyxin B Resistance in Klebsiella pneumoniae owing to a 42 bp Deletion in the Sequence of phoQ. BioMed Research International No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1135050
American Medical Association (AMA)
Xu, Qingqing& Xu, Teng& Zhuang, Yuan& Liu, Xiaofen& Li, Ying& Chen, Yijian. In Vivo Development of Polymyxin B Resistance in Klebsiella pneumoniae owing to a 42 bp Deletion in the Sequence of phoQ. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1135050
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135050