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Barcoding the Dendrobium (Orchidaceae) Species and Analysis of the Intragenomic Variation Based on the Internal Transcribed Spacer 2
المؤلفون المشاركون
Han, Jianping
Chen, Xiaochen
Wang, Xiaoyue
Yang, Pei
Wang, Lili
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-10-17
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Many species belonging to the genus Dendrobium are of great commercial value.
However, their difficult growth conditions and high demand have caused many of these species to become endangered.
Indeed, counterfeit Dendrobium products are common, especially in medicinal markets.
This study aims to assess the suitability of the internal transcribed spacer 2 (ITS2) region as a marker for identifying Dendrobium and to evaluate its intragenomic variation in Dendrobium species.
In total, 29,624 ITS2 copies from 18 species were obtained using 454 pyrosequencing to evaluate intragenomic variation.
In addition, 513 ITS2 sequences from 26 Dendrobium species were used to assess its identification suitability.
The highest intragenomic genetic distance was observed in Dendrobium chrysotoxum (0.081).
The average intraspecific genetic distances of each species ranged from 0 to 0.032.
Phylogenetic trees based on ITS2 sequences showed that most Dendrobium species are monophyletic.
The intragenomic and intraspecies divergence analysis showed that greater intragenomic divergence is mostly correlated with larger intraspecific variation.
As a major ITS2 variant becomes more common in genome, there are fewer intraspecific variable sites in ITS2 sequences at the species level.
The results demonstrated that the intragenomic multiple copies of ITS2 did not affect species identification.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Xiaoyue& Chen, Xiaochen& Yang, Pei& Wang, Lili& Han, Jianping. 2017. Barcoding the Dendrobium (Orchidaceae) Species and Analysis of the Intragenomic Variation Based on the Internal Transcribed Spacer 2. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1135095
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Xiaoyue…[et al.]. Barcoding the Dendrobium (Orchidaceae) Species and Analysis of the Intragenomic Variation Based on the Internal Transcribed Spacer 2. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1135095
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Xiaoyue& Chen, Xiaochen& Yang, Pei& Wang, Lili& Han, Jianping. Barcoding the Dendrobium (Orchidaceae) Species and Analysis of the Intragenomic Variation Based on the Internal Transcribed Spacer 2. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1135095
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1135095
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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