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Barcoding the Dendrobium (Orchidaceae) Species and Analysis of the Intragenomic Variation Based on the Internal Transcribed Spacer 2
Joint Authors
Han, Jianping
Chen, Xiaochen
Wang, Xiaoyue
Yang, Pei
Wang, Lili
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Many species belonging to the genus Dendrobium are of great commercial value.
However, their difficult growth conditions and high demand have caused many of these species to become endangered.
Indeed, counterfeit Dendrobium products are common, especially in medicinal markets.
This study aims to assess the suitability of the internal transcribed spacer 2 (ITS2) region as a marker for identifying Dendrobium and to evaluate its intragenomic variation in Dendrobium species.
In total, 29,624 ITS2 copies from 18 species were obtained using 454 pyrosequencing to evaluate intragenomic variation.
In addition, 513 ITS2 sequences from 26 Dendrobium species were used to assess its identification suitability.
The highest intragenomic genetic distance was observed in Dendrobium chrysotoxum (0.081).
The average intraspecific genetic distances of each species ranged from 0 to 0.032.
Phylogenetic trees based on ITS2 sequences showed that most Dendrobium species are monophyletic.
The intragenomic and intraspecies divergence analysis showed that greater intragenomic divergence is mostly correlated with larger intraspecific variation.
As a major ITS2 variant becomes more common in genome, there are fewer intraspecific variable sites in ITS2 sequences at the species level.
The results demonstrated that the intragenomic multiple copies of ITS2 did not affect species identification.
American Psychological Association (APA)
Wang, Xiaoyue& Chen, Xiaochen& Yang, Pei& Wang, Lili& Han, Jianping. 2017. Barcoding the Dendrobium (Orchidaceae) Species and Analysis of the Intragenomic Variation Based on the Internal Transcribed Spacer 2. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1135095
Modern Language Association (MLA)
Wang, Xiaoyue…[et al.]. Barcoding the Dendrobium (Orchidaceae) Species and Analysis of the Intragenomic Variation Based on the Internal Transcribed Spacer 2. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1135095
American Medical Association (AMA)
Wang, Xiaoyue& Chen, Xiaochen& Yang, Pei& Wang, Lili& Han, Jianping. Barcoding the Dendrobium (Orchidaceae) Species and Analysis of the Intragenomic Variation Based on the Internal Transcribed Spacer 2. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1135095
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135095