Phloretin Modulates Human Th17Treg Cell Differentiation In Vitro via AMPK Signaling
المؤلفون المشاركون
Jiao, Ao
Yang, Zhaoming
Fu, Xibo
Hua, Xiangdong
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-07-29
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Context.
The imbalance between T helper 17 (Th17) cell and regulatory T (Treg) cell is involved in many immune disorders and diseases.
Phloretin, a dihydrochalcone structural flavonoid compound, possesses many bioactive properties.
However, whether phloretin could impact on the differentiation of T cells is not completely clear.
Objective.
We conducted studies to explore the effect of phloretin on glucose uptake, proliferation, and differentiation of human peripheral blood CD4+ T cells and investigated the mechanism of phloretin on inducing Th17/Treg development.
Methods.
Naïve CD4+ T cells were purified from peripheral blood of healthy volunteers, stimulated with anti-CD3/CD28 antibodies, and polarized in vitro to generate Th17 or Treg cells.
Glucose uptake, proliferation, cell cycle, protein expression (phospho-Stat3, phospho-Stat5), and Th17 and Treg cell numbers were analyzed by flow cytometry.
AMP-activated protein kinase (AMPK) signaling was analyzed by western blot.
Results and Discussion.
Phloretin could inhibit the glucose uptake and proliferation of activated CD4+ T cells.
The proliferation inhibition was due to the G0/G1 phase arrest.
Phloretin decreased Th17 cell generation and phospho-Stat3 expression as well as increased Treg cell generation and phospho-Stat5 expression in the process of inducing Th17/Treg differentiation.
The phosphorylation level of AMPK was significantly enhanced, while the phosphorylation level of mTOR was significantly decreased in activated CD4+ T cells under phloretin treatment.
The AMPK signaling inhibitor compound C (Com C) could neutralize the effect of phloretin, while the agonist 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) could impact the Th17/Treg balance similar to phloretin during Th17/Treg induction.
Conclusion.
Our results suggest that phloretin can mediate the Th17/Treg balance by regulating metabolism via the AMPK signal pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jiao, Ao& Yang, Zhaoming& Fu, Xibo& Hua, Xiangdong. 2020. Phloretin Modulates Human Th17Treg Cell Differentiation In Vitro via AMPK Signaling. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1135697
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jiao, Ao…[et al.]. Phloretin Modulates Human Th17Treg Cell Differentiation In Vitro via AMPK Signaling. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1135697
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jiao, Ao& Yang, Zhaoming& Fu, Xibo& Hua, Xiangdong. Phloretin Modulates Human Th17Treg Cell Differentiation In Vitro via AMPK Signaling. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1135697
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1135697
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر