Phloretin Modulates Human Th17Treg Cell Differentiation In Vitro via AMPK Signaling
Joint Authors
Jiao, Ao
Yang, Zhaoming
Fu, Xibo
Hua, Xiangdong
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-29
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Context.
The imbalance between T helper 17 (Th17) cell and regulatory T (Treg) cell is involved in many immune disorders and diseases.
Phloretin, a dihydrochalcone structural flavonoid compound, possesses many bioactive properties.
However, whether phloretin could impact on the differentiation of T cells is not completely clear.
Objective.
We conducted studies to explore the effect of phloretin on glucose uptake, proliferation, and differentiation of human peripheral blood CD4+ T cells and investigated the mechanism of phloretin on inducing Th17/Treg development.
Methods.
Naïve CD4+ T cells were purified from peripheral blood of healthy volunteers, stimulated with anti-CD3/CD28 antibodies, and polarized in vitro to generate Th17 or Treg cells.
Glucose uptake, proliferation, cell cycle, protein expression (phospho-Stat3, phospho-Stat5), and Th17 and Treg cell numbers were analyzed by flow cytometry.
AMP-activated protein kinase (AMPK) signaling was analyzed by western blot.
Results and Discussion.
Phloretin could inhibit the glucose uptake and proliferation of activated CD4+ T cells.
The proliferation inhibition was due to the G0/G1 phase arrest.
Phloretin decreased Th17 cell generation and phospho-Stat3 expression as well as increased Treg cell generation and phospho-Stat5 expression in the process of inducing Th17/Treg differentiation.
The phosphorylation level of AMPK was significantly enhanced, while the phosphorylation level of mTOR was significantly decreased in activated CD4+ T cells under phloretin treatment.
The AMPK signaling inhibitor compound C (Com C) could neutralize the effect of phloretin, while the agonist 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) could impact the Th17/Treg balance similar to phloretin during Th17/Treg induction.
Conclusion.
Our results suggest that phloretin can mediate the Th17/Treg balance by regulating metabolism via the AMPK signal pathway.
American Psychological Association (APA)
Jiao, Ao& Yang, Zhaoming& Fu, Xibo& Hua, Xiangdong. 2020. Phloretin Modulates Human Th17Treg Cell Differentiation In Vitro via AMPK Signaling. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1135697
Modern Language Association (MLA)
Jiao, Ao…[et al.]. Phloretin Modulates Human Th17Treg Cell Differentiation In Vitro via AMPK Signaling. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1135697
American Medical Association (AMA)
Jiao, Ao& Yang, Zhaoming& Fu, Xibo& Hua, Xiangdong. Phloretin Modulates Human Th17Treg Cell Differentiation In Vitro via AMPK Signaling. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1135697
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135697