The Effect of Different Thawing Rates on Cryopreserved Human Iliac Arteries Allograft’s Structural Damage and Mechanical Properties
المؤلفون المشاركون
Novotny, Robert
Mericka, Pavel
Chlupac, Jaroslav
Matejka, Roman
Kristek, Jakub
Marada, Tomas
Konarik, Miroslav
Ivak, Peter
Sterba, Lubomir
Hlubocky, Jaroslav
Pirk, Jan
Janousek, Libor
Fronek, Jiri
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-10-08
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Introduction.
The rate of thawing of cryopreserved human iliac arteries allografts (CHIAA) directly affects the severeness of structural changes that occur during this process.
Method.
The experiment was performed on ten CHIAA.
The 10% dimethylsulphoxide in 6% hydroxyethyl starch solution was used as the cryoprotectant; all CHIAA were cooled at a controlled rate and stored in the vapor phase of liquid nitrogen (-194°C).
Two thawing protocols were tested: (1) placing the CHIAA in a water bath at 37°C, and (2) the CHIAA were thawed in a controlled environment at 5°C.
All samples underwent analysis under a scanning electron microscope.
Testing of the mechanical properties of the CHIAA was evaluated on a custom-built single axis strain testing machine.
Longitudinal and circumferential samples were prepared from each tested CHIAA.
Results.
Ultrastructural analysis revealed that all five CHIAA thawed during the thawing protocol 1 which showed significantly more damage to the subendothelial structures when compared to the samples thawed in protocol 2.
Mechanical properties: Thawing protocol 1—longitudinal UTS 2,53±0,47 MPa at relative strain 1,27±0,12 and circumferential UTS 1,94±0,27 MPa at relative strain 1,33±0,09.
Thawing protocol 2—longitudinal ultimate tensile strain (UTS) 2,42±0,34 MPa at relative strain 1,32±0,09 and circumferential UTS 1,98±0,26 MPa at relative strain 1,29±0,07.
Comparing UTS showed no statistical difference between thawing methods.
Conclusion.
Despite the significant differences in structural changes of presented thawing protocols, the ultimate tensile strain showed no statistical difference between thawing methods.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Novotny, Robert& Mericka, Pavel& Chlupac, Jaroslav& Matejka, Roman& Kristek, Jakub& Marada, Tomas…[et al.]. 2020. The Effect of Different Thawing Rates on Cryopreserved Human Iliac Arteries Allograft’s Structural Damage and Mechanical Properties. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1135999
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Novotny, Robert…[et al.]. The Effect of Different Thawing Rates on Cryopreserved Human Iliac Arteries Allograft’s Structural Damage and Mechanical Properties. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1135999
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Novotny, Robert& Mericka, Pavel& Chlupac, Jaroslav& Matejka, Roman& Kristek, Jakub& Marada, Tomas…[et al.]. The Effect of Different Thawing Rates on Cryopreserved Human Iliac Arteries Allograft’s Structural Damage and Mechanical Properties. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1135999
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1135999
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر