The Effect of Different Thawing Rates on Cryopreserved Human Iliac Arteries Allograft’s Structural Damage and Mechanical Properties
Joint Authors
Novotny, Robert
Mericka, Pavel
Chlupac, Jaroslav
Matejka, Roman
Kristek, Jakub
Marada, Tomas
Konarik, Miroslav
Ivak, Peter
Sterba, Lubomir
Hlubocky, Jaroslav
Pirk, Jan
Janousek, Libor
Fronek, Jiri
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-08
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Introduction.
The rate of thawing of cryopreserved human iliac arteries allografts (CHIAA) directly affects the severeness of structural changes that occur during this process.
Method.
The experiment was performed on ten CHIAA.
The 10% dimethylsulphoxide in 6% hydroxyethyl starch solution was used as the cryoprotectant; all CHIAA were cooled at a controlled rate and stored in the vapor phase of liquid nitrogen (-194°C).
Two thawing protocols were tested: (1) placing the CHIAA in a water bath at 37°C, and (2) the CHIAA were thawed in a controlled environment at 5°C.
All samples underwent analysis under a scanning electron microscope.
Testing of the mechanical properties of the CHIAA was evaluated on a custom-built single axis strain testing machine.
Longitudinal and circumferential samples were prepared from each tested CHIAA.
Results.
Ultrastructural analysis revealed that all five CHIAA thawed during the thawing protocol 1 which showed significantly more damage to the subendothelial structures when compared to the samples thawed in protocol 2.
Mechanical properties: Thawing protocol 1—longitudinal UTS 2,53±0,47 MPa at relative strain 1,27±0,12 and circumferential UTS 1,94±0,27 MPa at relative strain 1,33±0,09.
Thawing protocol 2—longitudinal ultimate tensile strain (UTS) 2,42±0,34 MPa at relative strain 1,32±0,09 and circumferential UTS 1,98±0,26 MPa at relative strain 1,29±0,07.
Comparing UTS showed no statistical difference between thawing methods.
Conclusion.
Despite the significant differences in structural changes of presented thawing protocols, the ultimate tensile strain showed no statistical difference between thawing methods.
American Psychological Association (APA)
Novotny, Robert& Mericka, Pavel& Chlupac, Jaroslav& Matejka, Roman& Kristek, Jakub& Marada, Tomas…[et al.]. 2020. The Effect of Different Thawing Rates on Cryopreserved Human Iliac Arteries Allograft’s Structural Damage and Mechanical Properties. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1135999
Modern Language Association (MLA)
Novotny, Robert…[et al.]. The Effect of Different Thawing Rates on Cryopreserved Human Iliac Arteries Allograft’s Structural Damage and Mechanical Properties. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1135999
American Medical Association (AMA)
Novotny, Robert& Mericka, Pavel& Chlupac, Jaroslav& Matejka, Roman& Kristek, Jakub& Marada, Tomas…[et al.]. The Effect of Different Thawing Rates on Cryopreserved Human Iliac Arteries Allograft’s Structural Damage and Mechanical Properties. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1135999
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135999