Curcumin Improves the Renal Autophagy in Rat Experimental Membranous Nephropathy via Regulating the PI3KAKTmTOR and Nrf2HO-1 Signaling Pathways
المؤلفون المشاركون
Ren, Yan
Hu, Xiao
Zhao, Li
Jin, Juan
Di Tu, Qiu
He, Qiang
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-11-02
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Membranous nephropathy (MN, also known as membranous glomerulopathy) is one of the many glomerular diseases causing nephrotic syndrome.
The literature indicates that autophagy is associated with the homeostasis of podocytes in glomeruli.
Curcumin, the main active component in turmeric, has drawn attention for its effective bioactivities against chronic kidney disease.
The current study was aimed at assessing the effects of curcumin and exploring the underlying mechanism that mediates autophagy in an animal model of passive Heymann nephritis (PHN) in rats.
Passive Heymann nephritis (PHN) was induced in male SD rats by intraperitoneal injection of anti-Fx1A serum.
The rats were divided into 3 groups: control (n=10, normal diet), model group (n=10, 0.5% sodium carboxymethylcellulose), and curcumin (n=10, 300 mg/kg/d).
The kidney function and oxidative stress indicators were measured using commercial diagnostic kits, and the histomorphology of renal tissues was observed.
The number of podocytes was measured by immunohistochemistry.
Meanwhile, the autophagosomes in podocyte were analyzed by transmission electron microscopy and the immunofluorescence assay pointing to p62, an autophagic marker.
Western blot analyzed the levels of apoptosis, autophagy, PI3K/AKT/mTOR, and Nrf2/HO-1 pathway-associated proteins.
The total cholesterol (TC), triglycerides (TG), creatinine (Scr), blood urea nitrogen (BUN), urine volume, and urine albumin of PHN rats were significantly reduced by the administration of curcumin and attenuated renal histomorphological changes in model rats.
Meanwhile, curcumin improved the oxidative stress response by decreasing MDA and increasing SOD, GSH, and CAT levels in the kidney of PHN rats.
Furthermore, curcumin significantly ameliorated the podocyte loss, along with the fusion, and increased the autophagic vacuoles compared to the PHN control rats.
In addition, curcumin downregulated the expression of Bax, Caspase-3, p62, PI3K, p-AKT, and p-mTOR proteins and upregulated the Bcl-2, beclin1, LC3, Nrf2, and HO-1 levels in this animal model.
The results provide a scientific basis that curcumin could significantly alleviate the development of MN by inducing autophagy and alleviating renal oxidative stress through the PI3K/AKT/mTOR and Nrf2/HO-1 pathways.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Di Tu, Qiu& Jin, Juan& Hu, Xiao& Ren, Yan& Zhao, Li& He, Qiang. 2020. Curcumin Improves the Renal Autophagy in Rat Experimental Membranous Nephropathy via Regulating the PI3KAKTmTOR and Nrf2HO-1 Signaling Pathways. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1136453
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Di Tu, Qiu…[et al.]. Curcumin Improves the Renal Autophagy in Rat Experimental Membranous Nephropathy via Regulating the PI3KAKTmTOR and Nrf2HO-1 Signaling Pathways. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1136453
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Di Tu, Qiu& Jin, Juan& Hu, Xiao& Ren, Yan& Zhao, Li& He, Qiang. Curcumin Improves the Renal Autophagy in Rat Experimental Membranous Nephropathy via Regulating the PI3KAKTmTOR and Nrf2HO-1 Signaling Pathways. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1136453
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1136453
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر