Curcumin Improves the Renal Autophagy in Rat Experimental Membranous Nephropathy via Regulating the PI3KAKTmTOR and Nrf2HO-1 Signaling Pathways
Joint Authors
Ren, Yan
Hu, Xiao
Zhao, Li
Jin, Juan
Di Tu, Qiu
He, Qiang
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-02
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Membranous nephropathy (MN, also known as membranous glomerulopathy) is one of the many glomerular diseases causing nephrotic syndrome.
The literature indicates that autophagy is associated with the homeostasis of podocytes in glomeruli.
Curcumin, the main active component in turmeric, has drawn attention for its effective bioactivities against chronic kidney disease.
The current study was aimed at assessing the effects of curcumin and exploring the underlying mechanism that mediates autophagy in an animal model of passive Heymann nephritis (PHN) in rats.
Passive Heymann nephritis (PHN) was induced in male SD rats by intraperitoneal injection of anti-Fx1A serum.
The rats were divided into 3 groups: control (n=10, normal diet), model group (n=10, 0.5% sodium carboxymethylcellulose), and curcumin (n=10, 300 mg/kg/d).
The kidney function and oxidative stress indicators were measured using commercial diagnostic kits, and the histomorphology of renal tissues was observed.
The number of podocytes was measured by immunohistochemistry.
Meanwhile, the autophagosomes in podocyte were analyzed by transmission electron microscopy and the immunofluorescence assay pointing to p62, an autophagic marker.
Western blot analyzed the levels of apoptosis, autophagy, PI3K/AKT/mTOR, and Nrf2/HO-1 pathway-associated proteins.
The total cholesterol (TC), triglycerides (TG), creatinine (Scr), blood urea nitrogen (BUN), urine volume, and urine albumin of PHN rats were significantly reduced by the administration of curcumin and attenuated renal histomorphological changes in model rats.
Meanwhile, curcumin improved the oxidative stress response by decreasing MDA and increasing SOD, GSH, and CAT levels in the kidney of PHN rats.
Furthermore, curcumin significantly ameliorated the podocyte loss, along with the fusion, and increased the autophagic vacuoles compared to the PHN control rats.
In addition, curcumin downregulated the expression of Bax, Caspase-3, p62, PI3K, p-AKT, and p-mTOR proteins and upregulated the Bcl-2, beclin1, LC3, Nrf2, and HO-1 levels in this animal model.
The results provide a scientific basis that curcumin could significantly alleviate the development of MN by inducing autophagy and alleviating renal oxidative stress through the PI3K/AKT/mTOR and Nrf2/HO-1 pathways.
American Psychological Association (APA)
Di Tu, Qiu& Jin, Juan& Hu, Xiao& Ren, Yan& Zhao, Li& He, Qiang. 2020. Curcumin Improves the Renal Autophagy in Rat Experimental Membranous Nephropathy via Regulating the PI3KAKTmTOR and Nrf2HO-1 Signaling Pathways. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1136453
Modern Language Association (MLA)
Di Tu, Qiu…[et al.]. Curcumin Improves the Renal Autophagy in Rat Experimental Membranous Nephropathy via Regulating the PI3KAKTmTOR and Nrf2HO-1 Signaling Pathways. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1136453
American Medical Association (AMA)
Di Tu, Qiu& Jin, Juan& Hu, Xiao& Ren, Yan& Zhao, Li& He, Qiang. Curcumin Improves the Renal Autophagy in Rat Experimental Membranous Nephropathy via Regulating the PI3KAKTmTOR and Nrf2HO-1 Signaling Pathways. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1136453
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136453