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Preparation and Nanoencapsulation of Lectin from Lepidium sativum on Chitosan-Tripolyphosphate Nanoparticle and Their Cytotoxicity against Hepatocellular Carcinoma Cells (HepG2)
المؤلفون المشاركون
Yasin, Unzila
Bilal, Muhammad
Bashir, Hamid
Amirzada, Muhammad Imran
Sumrin, Aleena
Mehmood, Rubada
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-10-22
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Lectins are the oligomeric sugar-specific glycoprotein of nonimmune origin, are involved in the multiple biological recognition process, and have the capacity to perform a wide variety of physiological functions including antifungal, antiviral, antitumor, and cell agglutination.
The main objective of the current study was to prepare lectin protein-loaded chitosan-TPP nanoparticles via ionic gelation methods with different CS/TPP ratios and to investigate anticancer potential against HepG2 cells.
The best ratio showed the mean particle size (298.10±1.9 nm, 21.05±0.95 mv) with optimal encapsulation efficiencies of 52.435±0.09%.
The cytotoxicity was evaluated against HepG2 cells, and IC50 values obtained were 265 μg/ml for lectin protein and 105 μg/ml for lectin-loaded chitosan-TPP nanoparticles, respectively.
The mRNA expression of proliferation markers like GPC3 was significantly decreased in hepatocellular carcinoma cells (HepG2) during lectin protein-loaded chitosan-TPP nanoparticle treatment.
Apoptotic genes that indicating a marked increase in expression are Caspase 3, p53, and Bax, while Bcl2 and AFP showed a downregulation of expression after treatment of HepG2 cells with lectin-loaded chitosan-TPP nanoparticles.
The preliminary findings of our study highlighted that lectin protein-loaded chitosan-TPP nanoparticles could be a promising anticancer agent.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yasin, Unzila& Bilal, Muhammad& Bashir, Hamid& Amirzada, Muhammad Imran& Sumrin, Aleena& Mehmood, Rubada. 2020. Preparation and Nanoencapsulation of Lectin from Lepidium sativum on Chitosan-Tripolyphosphate Nanoparticle and Their Cytotoxicity against Hepatocellular Carcinoma Cells (HepG2). BioMed Research International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1136689
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yasin, Unzila…[et al.]. Preparation and Nanoencapsulation of Lectin from Lepidium sativum on Chitosan-Tripolyphosphate Nanoparticle and Their Cytotoxicity against Hepatocellular Carcinoma Cells (HepG2). BioMed Research International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1136689
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yasin, Unzila& Bilal, Muhammad& Bashir, Hamid& Amirzada, Muhammad Imran& Sumrin, Aleena& Mehmood, Rubada. Preparation and Nanoencapsulation of Lectin from Lepidium sativum on Chitosan-Tripolyphosphate Nanoparticle and Their Cytotoxicity against Hepatocellular Carcinoma Cells (HepG2). BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1136689
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1136689
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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