Preparation and Nanoencapsulation of Lectin from Lepidium sativum on Chitosan-Tripolyphosphate Nanoparticle and Their Cytotoxicity against Hepatocellular Carcinoma Cells (HepG2)
Joint Authors
Yasin, Unzila
Bilal, Muhammad
Bashir, Hamid
Amirzada, Muhammad Imran
Sumrin, Aleena
Mehmood, Rubada
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Lectins are the oligomeric sugar-specific glycoprotein of nonimmune origin, are involved in the multiple biological recognition process, and have the capacity to perform a wide variety of physiological functions including antifungal, antiviral, antitumor, and cell agglutination.
The main objective of the current study was to prepare lectin protein-loaded chitosan-TPP nanoparticles via ionic gelation methods with different CS/TPP ratios and to investigate anticancer potential against HepG2 cells.
The best ratio showed the mean particle size (298.10±1.9 nm, 21.05±0.95 mv) with optimal encapsulation efficiencies of 52.435±0.09%.
The cytotoxicity was evaluated against HepG2 cells, and IC50 values obtained were 265 μg/ml for lectin protein and 105 μg/ml for lectin-loaded chitosan-TPP nanoparticles, respectively.
The mRNA expression of proliferation markers like GPC3 was significantly decreased in hepatocellular carcinoma cells (HepG2) during lectin protein-loaded chitosan-TPP nanoparticle treatment.
Apoptotic genes that indicating a marked increase in expression are Caspase 3, p53, and Bax, while Bcl2 and AFP showed a downregulation of expression after treatment of HepG2 cells with lectin-loaded chitosan-TPP nanoparticles.
The preliminary findings of our study highlighted that lectin protein-loaded chitosan-TPP nanoparticles could be a promising anticancer agent.
American Psychological Association (APA)
Yasin, Unzila& Bilal, Muhammad& Bashir, Hamid& Amirzada, Muhammad Imran& Sumrin, Aleena& Mehmood, Rubada. 2020. Preparation and Nanoencapsulation of Lectin from Lepidium sativum on Chitosan-Tripolyphosphate Nanoparticle and Their Cytotoxicity against Hepatocellular Carcinoma Cells (HepG2). BioMed Research International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1136689
Modern Language Association (MLA)
Yasin, Unzila…[et al.]. Preparation and Nanoencapsulation of Lectin from Lepidium sativum on Chitosan-Tripolyphosphate Nanoparticle and Their Cytotoxicity against Hepatocellular Carcinoma Cells (HepG2). BioMed Research International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1136689
American Medical Association (AMA)
Yasin, Unzila& Bilal, Muhammad& Bashir, Hamid& Amirzada, Muhammad Imran& Sumrin, Aleena& Mehmood, Rubada. Preparation and Nanoencapsulation of Lectin from Lepidium sativum on Chitosan-Tripolyphosphate Nanoparticle and Their Cytotoxicity against Hepatocellular Carcinoma Cells (HepG2). BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1136689
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136689