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Ganab Haploinsufficiency Does Not Cause Polycystic Kidney Disease or Polycystic Liver Disease in Mice
المؤلفون المشاركون
Chen, Xiangmei
Cai, Guangyan
Geng, Guangrui
Xiao, Yunming
Zhang, Yingjie
Shen, Wanjun
Liu, Jiaona
Zhu, Fei
Wang, Xu
Wu, Jie
Liu, Ran
Bai, Xueyuan
Li, Qinggang
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-05-20
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Background.
Heterozygous GANAB mutations that can cause autosomal dominant polycystic kidney disease (ADPKD) and polycystic liver disease (PLD) have been described previously, but their roles in ADPKD and PLD are largely unknown.
With the increase in polycystic kidney disease caused by GANAB gene mutations in recent years, a suitable animal model is still needed to further explore the pathogenic role of this gene.
Methods.
To construct a mouse model of Ganab gene deletion, we analyzed the Ganab gene structure and designed two CRISPR-/Cas9-based targeting strategies.
The Cas9/sgRNA we constructed was microinjected into fertilized mouse eggs to obtain chimeric F0 mice.
Mice with stable genotypes were selected from offspring born after mating F0 mice with wild-type mice.
Results.
We found that homozygous mutation of the Ganab gene in C57BL/6 mice resulted in early embryonic lethality, and there were no cysts in the kidneys or livers of Ganab+/- mice.
Additionally, Ganab protein expression was reduced by at least 50%, while the expression of ADPKD proteins (PC1 and PC2) and acetylated tubulin was not affected in the Ganab+/- kidney.
However, the Ganab+/- mice did not show any abnormal clinical phenotypes after birth and failed to reveal renal tubule dilatation or any abnormalities of the glomeruli in the Ganab+/- kidney.
Conclusions.
Homozygous Ganab mutations are lethal in the fetal stage, and Ganab haploinsufficiency does not cause kidney or liver cysts in mice, suggesting that it may not be the causative gene in polycystic kidney disease.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Geng, Guangrui& Xiao, Yunming& Zhang, Yingjie& Shen, Wanjun& Liu, Jiaona& Zhu, Fei…[et al.]. 2020. Ganab Haploinsufficiency Does Not Cause Polycystic Kidney Disease or Polycystic Liver Disease in Mice. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1136900
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Geng, Guangrui…[et al.]. Ganab Haploinsufficiency Does Not Cause Polycystic Kidney Disease or Polycystic Liver Disease in Mice. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1136900
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Geng, Guangrui& Xiao, Yunming& Zhang, Yingjie& Shen, Wanjun& Liu, Jiaona& Zhu, Fei…[et al.]. Ganab Haploinsufficiency Does Not Cause Polycystic Kidney Disease or Polycystic Liver Disease in Mice. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1136900
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1136900
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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