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Ganab Haploinsufficiency Does Not Cause Polycystic Kidney Disease or Polycystic Liver Disease in Mice
Joint Authors
Chen, Xiangmei
Cai, Guangyan
Geng, Guangrui
Xiao, Yunming
Zhang, Yingjie
Shen, Wanjun
Liu, Jiaona
Zhu, Fei
Wang, Xu
Wu, Jie
Liu, Ran
Bai, Xueyuan
Li, Qinggang
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-20
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Heterozygous GANAB mutations that can cause autosomal dominant polycystic kidney disease (ADPKD) and polycystic liver disease (PLD) have been described previously, but their roles in ADPKD and PLD are largely unknown.
With the increase in polycystic kidney disease caused by GANAB gene mutations in recent years, a suitable animal model is still needed to further explore the pathogenic role of this gene.
Methods.
To construct a mouse model of Ganab gene deletion, we analyzed the Ganab gene structure and designed two CRISPR-/Cas9-based targeting strategies.
The Cas9/sgRNA we constructed was microinjected into fertilized mouse eggs to obtain chimeric F0 mice.
Mice with stable genotypes were selected from offspring born after mating F0 mice with wild-type mice.
Results.
We found that homozygous mutation of the Ganab gene in C57BL/6 mice resulted in early embryonic lethality, and there were no cysts in the kidneys or livers of Ganab+/- mice.
Additionally, Ganab protein expression was reduced by at least 50%, while the expression of ADPKD proteins (PC1 and PC2) and acetylated tubulin was not affected in the Ganab+/- kidney.
However, the Ganab+/- mice did not show any abnormal clinical phenotypes after birth and failed to reveal renal tubule dilatation or any abnormalities of the glomeruli in the Ganab+/- kidney.
Conclusions.
Homozygous Ganab mutations are lethal in the fetal stage, and Ganab haploinsufficiency does not cause kidney or liver cysts in mice, suggesting that it may not be the causative gene in polycystic kidney disease.
American Psychological Association (APA)
Geng, Guangrui& Xiao, Yunming& Zhang, Yingjie& Shen, Wanjun& Liu, Jiaona& Zhu, Fei…[et al.]. 2020. Ganab Haploinsufficiency Does Not Cause Polycystic Kidney Disease or Polycystic Liver Disease in Mice. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1136900
Modern Language Association (MLA)
Geng, Guangrui…[et al.]. Ganab Haploinsufficiency Does Not Cause Polycystic Kidney Disease or Polycystic Liver Disease in Mice. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1136900
American Medical Association (AMA)
Geng, Guangrui& Xiao, Yunming& Zhang, Yingjie& Shen, Wanjun& Liu, Jiaona& Zhu, Fei…[et al.]. Ganab Haploinsufficiency Does Not Cause Polycystic Kidney Disease or Polycystic Liver Disease in Mice. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1136900
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136900