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Basic Fibroblast Growth Factor-Anchored Multilayered Mesenchymal Cell Sheets Accelerate Periosteal Bone Formation
المؤلفون المشاركون
Uchida, Kentaro
Takano, Shotaro
Sekiguchi, Hiroyuki
Fujimaki, Hisako
Matsushita, Osamu
Horikawa, Kyosuke
Saito, Wataru
Miyagi, Masayuki
Takaso, Masashi
Inoue, Gen
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-07-06
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Cell-based regenerative therapy has the potential to repair bone injuries or large defects that are recalcitrant to conventional treatment methods, including drugs and surgery.
Here, we developed a multilayered cell-based bone formation system using cells coated with fibronectin-gelatin (FN-G) nanofilms.
The multilayered mesenchymal cells (MLMCs) were formed after two days of culture and were shown to express higher levels of BMP-2 and VEGF compared to monolayer cultures of MCs.
The MLMCs were used as a graft material in combination with a fusion protein consisting of basic fibroblast growth factor (bFGF), polycystic kidney disease (PKD) domain, and the collagen-binding domain (CBD) of Clostridium histolyticum collagenase.
In femur sites grafted with the MLMCs, significantly higher levels of callus volume and bone mineral content were observed compared to the sham controls.
The callus volume and bone mineral content were further increased in femur sites grafted with bFGF-PKD-CBD/MLMCs.
Taken together, these results suggest that bFGF-PKD-CBD/MLMCs, which can be simply and rapidly generated in vitro, have the potential to promote bone repair when grafted into large defect sites.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Uchida, Kentaro& Inoue, Gen& Matsushita, Osamu& Horikawa, Kyosuke& Sekiguchi, Hiroyuki& Saito, Wataru…[et al.]. 2017. Basic Fibroblast Growth Factor-Anchored Multilayered Mesenchymal Cell Sheets Accelerate Periosteal Bone Formation. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1137037
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Uchida, Kentaro…[et al.]. Basic Fibroblast Growth Factor-Anchored Multilayered Mesenchymal Cell Sheets Accelerate Periosteal Bone Formation. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1137037
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Uchida, Kentaro& Inoue, Gen& Matsushita, Osamu& Horikawa, Kyosuke& Sekiguchi, Hiroyuki& Saito, Wataru…[et al.]. Basic Fibroblast Growth Factor-Anchored Multilayered Mesenchymal Cell Sheets Accelerate Periosteal Bone Formation. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1137037
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1137037
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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