Basic Fibroblast Growth Factor-Anchored Multilayered Mesenchymal Cell Sheets Accelerate Periosteal Bone Formation
Joint Authors
Uchida, Kentaro
Takano, Shotaro
Sekiguchi, Hiroyuki
Fujimaki, Hisako
Matsushita, Osamu
Horikawa, Kyosuke
Saito, Wataru
Miyagi, Masayuki
Takaso, Masashi
Inoue, Gen
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-06
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Cell-based regenerative therapy has the potential to repair bone injuries or large defects that are recalcitrant to conventional treatment methods, including drugs and surgery.
Here, we developed a multilayered cell-based bone formation system using cells coated with fibronectin-gelatin (FN-G) nanofilms.
The multilayered mesenchymal cells (MLMCs) were formed after two days of culture and were shown to express higher levels of BMP-2 and VEGF compared to monolayer cultures of MCs.
The MLMCs were used as a graft material in combination with a fusion protein consisting of basic fibroblast growth factor (bFGF), polycystic kidney disease (PKD) domain, and the collagen-binding domain (CBD) of Clostridium histolyticum collagenase.
In femur sites grafted with the MLMCs, significantly higher levels of callus volume and bone mineral content were observed compared to the sham controls.
The callus volume and bone mineral content were further increased in femur sites grafted with bFGF-PKD-CBD/MLMCs.
Taken together, these results suggest that bFGF-PKD-CBD/MLMCs, which can be simply and rapidly generated in vitro, have the potential to promote bone repair when grafted into large defect sites.
American Psychological Association (APA)
Uchida, Kentaro& Inoue, Gen& Matsushita, Osamu& Horikawa, Kyosuke& Sekiguchi, Hiroyuki& Saito, Wataru…[et al.]. 2017. Basic Fibroblast Growth Factor-Anchored Multilayered Mesenchymal Cell Sheets Accelerate Periosteal Bone Formation. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1137037
Modern Language Association (MLA)
Uchida, Kentaro…[et al.]. Basic Fibroblast Growth Factor-Anchored Multilayered Mesenchymal Cell Sheets Accelerate Periosteal Bone Formation. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1137037
American Medical Association (AMA)
Uchida, Kentaro& Inoue, Gen& Matsushita, Osamu& Horikawa, Kyosuke& Sekiguchi, Hiroyuki& Saito, Wataru…[et al.]. Basic Fibroblast Growth Factor-Anchored Multilayered Mesenchymal Cell Sheets Accelerate Periosteal Bone Formation. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1137037
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137037