CXXC5 Attenuates Pulmonary Fibrosis in a Bleomycin-Induced Mouse Model and MLFs by Suppression of the CD40CD40L Pathway
المؤلفون المشاركون
Cheng, Wei
Wang, Fangfang
Feng, Airan
Li, Xiaodan
Yu, Wencheng
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-04-04
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Objective.
To investigate the role of CXXC5 and the CD40/CD40L pathway in lung fibrosis.
Methods.
(1) We constructed mouse models of bleomycin-induced pulmonary fibrosis and transfected them with a CXXC5 overexpression vector to evaluate the severity of pulmonary fibrosis.
(2) Mouse lung fibroblast (MLF) models stably overexpressed or knockout of CXXC5 vector were constructed.
After transforming growth factor-β1 (TGF-β1) stimulation, we examined the proliferation and apoptosis of the MLF model and evaluated the expression of mesenchymal markers and the CXXC5/CD40/CD40L pathway.
Results.
(1) Compared with other groups, the overexpressed CXXC5 group had less alveolar structure destruction, thinner alveolar septum, and lower Ashcroft score.
(2) In bleomycin-induced mice, the expression of CD40 and CD40L increased at both transcriptional and protein levels, and the same changes were observed in α-smooth muscle actin (α-SMA) and collagen type I (Colla I).
After upregulation of CXXC5, the increase in CD40, CD40L, α-SMA, and Colla I was attenuated.
(3) Stimulated with TGF-β1, MLF proliferation was activated, apoptosis was suppressed, and the expression of CD40, CD40L, α-SMA, and Colla I was increased at both transcriptional and protein levels.
After upregulation of CXXC5, these changes were attenuated.
Conclusion.
CXXC5 inhibits pulmonary fibrosis and transformation to myofibroblasts by negative feedback regulation of the CD40/CD40L pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Cheng, Wei& Wang, Fangfang& Feng, Airan& Li, Xiaodan& Yu, Wencheng. 2020. CXXC5 Attenuates Pulmonary Fibrosis in a Bleomycin-Induced Mouse Model and MLFs by Suppression of the CD40CD40L Pathway. BioMed Research International،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1137193
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Cheng, Wei…[et al.]. CXXC5 Attenuates Pulmonary Fibrosis in a Bleomycin-Induced Mouse Model and MLFs by Suppression of the CD40CD40L Pathway. BioMed Research International No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1137193
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Cheng, Wei& Wang, Fangfang& Feng, Airan& Li, Xiaodan& Yu, Wencheng. CXXC5 Attenuates Pulmonary Fibrosis in a Bleomycin-Induced Mouse Model and MLFs by Suppression of the CD40CD40L Pathway. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1137193
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1137193
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر