CXXC5 Attenuates Pulmonary Fibrosis in a Bleomycin-Induced Mouse Model and MLFs by Suppression of the CD40CD40L Pathway
Joint Authors
Cheng, Wei
Wang, Fangfang
Feng, Airan
Li, Xiaodan
Yu, Wencheng
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-04
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Objective.
To investigate the role of CXXC5 and the CD40/CD40L pathway in lung fibrosis.
Methods.
(1) We constructed mouse models of bleomycin-induced pulmonary fibrosis and transfected them with a CXXC5 overexpression vector to evaluate the severity of pulmonary fibrosis.
(2) Mouse lung fibroblast (MLF) models stably overexpressed or knockout of CXXC5 vector were constructed.
After transforming growth factor-β1 (TGF-β1) stimulation, we examined the proliferation and apoptosis of the MLF model and evaluated the expression of mesenchymal markers and the CXXC5/CD40/CD40L pathway.
Results.
(1) Compared with other groups, the overexpressed CXXC5 group had less alveolar structure destruction, thinner alveolar septum, and lower Ashcroft score.
(2) In bleomycin-induced mice, the expression of CD40 and CD40L increased at both transcriptional and protein levels, and the same changes were observed in α-smooth muscle actin (α-SMA) and collagen type I (Colla I).
After upregulation of CXXC5, the increase in CD40, CD40L, α-SMA, and Colla I was attenuated.
(3) Stimulated with TGF-β1, MLF proliferation was activated, apoptosis was suppressed, and the expression of CD40, CD40L, α-SMA, and Colla I was increased at both transcriptional and protein levels.
After upregulation of CXXC5, these changes were attenuated.
Conclusion.
CXXC5 inhibits pulmonary fibrosis and transformation to myofibroblasts by negative feedback regulation of the CD40/CD40L pathway.
American Psychological Association (APA)
Cheng, Wei& Wang, Fangfang& Feng, Airan& Li, Xiaodan& Yu, Wencheng. 2020. CXXC5 Attenuates Pulmonary Fibrosis in a Bleomycin-Induced Mouse Model and MLFs by Suppression of the CD40CD40L Pathway. BioMed Research International،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1137193
Modern Language Association (MLA)
Cheng, Wei…[et al.]. CXXC5 Attenuates Pulmonary Fibrosis in a Bleomycin-Induced Mouse Model and MLFs by Suppression of the CD40CD40L Pathway. BioMed Research International No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1137193
American Medical Association (AMA)
Cheng, Wei& Wang, Fangfang& Feng, Airan& Li, Xiaodan& Yu, Wencheng. CXXC5 Attenuates Pulmonary Fibrosis in a Bleomycin-Induced Mouse Model and MLFs by Suppression of the CD40CD40L Pathway. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1137193
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137193