Online Detection of Peroxidase Using 3D Printing, Active Magnetic Mixing, and Spectra Analysis

المؤلفون المشاركون

Bai, Shanshan
Gan, Chengqi
Cai, Gaozhe
Wang, Lei
Chen, Mingyong
Han, Qingan
Lin, Jianhan

المصدر

BioMed Research International

العدد

المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-8، 8ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2017-04-24

دولة النشر

مصر

عدد الصفحات

8

التخصصات الرئيسية

الطب البشري

الملخص EN

A new method for online detection of peroxidase (POD) using 3D printing, active magnetic mixing, fluidic control, and optical detection was developed and demonstrated in this study.

The proposed POD detection system consisted of a 3D printing and active magnetic mixing based fluidic chip for online catalytic reaction, an optical detector with a fluidic flow cell for quantitative determination of the final catalysate, and a single-chip microcontroller based controller for automatic control of two rotating magnetic fields and four precise peristaltic pumps.

Horseradish peroxidase (HRP) was used as research model and a linear relationship between the absorbance at the characteristic wavelength of 450 nm and the concentration of HRP of 1/4–1/128 μg mL−1 was obtained as A = 0.257ln(C) + 1.425 (R2 = 0.976).

For the HRP spiked pork tests, the recoveries of HRP ranged from 93.5% to 110.4%, indicating that this proposed system was capable of detecting HRP in real samples.

It has the potential to be extended for online detection of the activity of other enzymes and integration with ELISA method for biological and chemical analysis.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Bai, Shanshan& Gan, Chengqi& Cai, Gaozhe& Wang, Lei& Chen, Mingyong& Han, Qingan…[et al.]. 2017. Online Detection of Peroxidase Using 3D Printing, Active Magnetic Mixing, and Spectra Analysis. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1137386

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Bai, Shanshan…[et al.]. Online Detection of Peroxidase Using 3D Printing, Active Magnetic Mixing, and Spectra Analysis. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1137386

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Bai, Shanshan& Gan, Chengqi& Cai, Gaozhe& Wang, Lei& Chen, Mingyong& Han, Qingan…[et al.]. Online Detection of Peroxidase Using 3D Printing, Active Magnetic Mixing, and Spectra Analysis. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1137386

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1137386