Molecular Characterization, Structural Modeling, and Evaluation of Antimicrobial Activity of Basrai Thaumatin-Like Protein against Fungal Infection
المؤلفون المشاركون
Yasmin, Nusrat
Saleem, Mahjabeen
Naz, Mamoona
Gul, Roquyya
Rehman, Hafiz Muzzammel
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-08-10
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
A thaumatin-like protein gene from Basrai banana was cloned and expressed in Escherichia coli.
Amplified gene product was cloned into pTZ57R/T vector and subcloned into expression vector pET22b(+) and resulting pET22b-basrai TLP construct was introduced into E.
coli BL21.
Maximum protein expression was obtained at 0.7 mM IPTG concentration after 6 hours at 37°C.
Western blot analysis showed the presence of approximately 20 kDa protein in induced cells.
Basrai antifungal TLP was tried as pharmacological agent against fungal disease.
Independently Basrai antifungal protein and amphotericin B exhibited their antifungal activity against A.
fumigatus; however combined effect of both agents maximized activity against the pathogen.
Docking studies were performed to evaluate the antimicrobial potential of TLP against A.
fumigatus by probing binding pattern of antifungal protein with plasma membrane ergosterol of targeted fungal strain.
Ice crystallization primarily damages frozen food items; however addition of antifreeze proteins limits the growth of ice crystal in frozen foods.
The potential of Basrai TLP protein, as an antifreezing agent, in controlling the ice crystal formation in frozen yogurt was also studied.
The scope of this study ranges from cost effective production of pharmaceutics to antifreezing and food preserving agent as well as other real life applications.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yasmin, Nusrat& Saleem, Mahjabeen& Naz, Mamoona& Gul, Roquyya& Rehman, Hafiz Muzzammel. 2017. Molecular Characterization, Structural Modeling, and Evaluation of Antimicrobial Activity of Basrai Thaumatin-Like Protein against Fungal Infection. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1137396
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yasmin, Nusrat…[et al.]. Molecular Characterization, Structural Modeling, and Evaluation of Antimicrobial Activity of Basrai Thaumatin-Like Protein against Fungal Infection. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1137396
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yasmin, Nusrat& Saleem, Mahjabeen& Naz, Mamoona& Gul, Roquyya& Rehman, Hafiz Muzzammel. Molecular Characterization, Structural Modeling, and Evaluation of Antimicrobial Activity of Basrai Thaumatin-Like Protein against Fungal Infection. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1137396
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1137396
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر