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Molecular Characterization, Structural Modeling, and Evaluation of Antimicrobial Activity of Basrai Thaumatin-Like Protein against Fungal Infection
Joint Authors
Yasmin, Nusrat
Saleem, Mahjabeen
Naz, Mamoona
Gul, Roquyya
Rehman, Hafiz Muzzammel
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
A thaumatin-like protein gene from Basrai banana was cloned and expressed in Escherichia coli.
Amplified gene product was cloned into pTZ57R/T vector and subcloned into expression vector pET22b(+) and resulting pET22b-basrai TLP construct was introduced into E.
coli BL21.
Maximum protein expression was obtained at 0.7 mM IPTG concentration after 6 hours at 37°C.
Western blot analysis showed the presence of approximately 20 kDa protein in induced cells.
Basrai antifungal TLP was tried as pharmacological agent against fungal disease.
Independently Basrai antifungal protein and amphotericin B exhibited their antifungal activity against A.
fumigatus; however combined effect of both agents maximized activity against the pathogen.
Docking studies were performed to evaluate the antimicrobial potential of TLP against A.
fumigatus by probing binding pattern of antifungal protein with plasma membrane ergosterol of targeted fungal strain.
Ice crystallization primarily damages frozen food items; however addition of antifreeze proteins limits the growth of ice crystal in frozen foods.
The potential of Basrai TLP protein, as an antifreezing agent, in controlling the ice crystal formation in frozen yogurt was also studied.
The scope of this study ranges from cost effective production of pharmaceutics to antifreezing and food preserving agent as well as other real life applications.
American Psychological Association (APA)
Yasmin, Nusrat& Saleem, Mahjabeen& Naz, Mamoona& Gul, Roquyya& Rehman, Hafiz Muzzammel. 2017. Molecular Characterization, Structural Modeling, and Evaluation of Antimicrobial Activity of Basrai Thaumatin-Like Protein against Fungal Infection. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1137396
Modern Language Association (MLA)
Yasmin, Nusrat…[et al.]. Molecular Characterization, Structural Modeling, and Evaluation of Antimicrobial Activity of Basrai Thaumatin-Like Protein against Fungal Infection. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1137396
American Medical Association (AMA)
Yasmin, Nusrat& Saleem, Mahjabeen& Naz, Mamoona& Gul, Roquyya& Rehman, Hafiz Muzzammel. Molecular Characterization, Structural Modeling, and Evaluation of Antimicrobial Activity of Basrai Thaumatin-Like Protein against Fungal Infection. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1137396
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137396