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Structure and Function of Trypsin-Loaded Fibrinolytic Liposomes
المؤلفون المشاركون
Bóta, Attila
Tanka-Salamon, Anna
Wacha, András
Mihály, Judith
Lovas, Miklós
Kolev, Krasimir
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-07-03
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Protease encapsulation and its targeted release in thrombi may contribute to the reduction of haemorrhagic complications of thrombolysis.
We aimed to prepare sterically stabilized trypsin-loaded liposomes (SSLT) and characterize their structure and fibrinolytic efficiency.
Hydrogenated soybean phosphatidylcholine-based SSLT were prepared and their structure was studied by transmission electron microscopy combined with freeze fracture (FF-TEM), Fourier transform infrared spectroscopy (FT-IR), and small-angle X-ray scattering (SAXS).
Fibrinolytic activity was examined at 45, 37, or 24°C on fibrin or plasma clots with turbidimetric and permeation-driven lysis assays.
Trypsin was shown to be attached to the inner surface of vesicles (SAXS and FF-TEM) close to the lipid hydrophilic/hydrophobic interface (FT-IR).
The thermosensitivity of SSLT was evidenced by enhanced fibrinolysis at 45°C: time to reduce the maximal turbidity to 20% decreased by 8.6% compared to 37°C and fibrin degradation product concentration in the permeation lysis assay was 2-fold to 5-fold higher than that at 24°C.
SSLT exerted its fibrinolytic action on fibrin clots under both static and dynamic conditions, whereas plasma clot dissolution was observed only in the permeation-driven assay.
The improved fibrinolytic efficiency of SSLT under dynamic conditions suggests that they may serve as a novel therapeutic candidate for dissolution of intravascular thrombi, which are typically exposed to permeation forces.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tanka-Salamon, Anna& Bóta, Attila& Wacha, András& Mihály, Judith& Lovas, Miklós& Kolev, Krasimir. 2017. Structure and Function of Trypsin-Loaded Fibrinolytic Liposomes. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1137439
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tanka-Salamon, Anna…[et al.]. Structure and Function of Trypsin-Loaded Fibrinolytic Liposomes. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1137439
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tanka-Salamon, Anna& Bóta, Attila& Wacha, András& Mihály, Judith& Lovas, Miklós& Kolev, Krasimir. Structure and Function of Trypsin-Loaded Fibrinolytic Liposomes. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1137439
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1137439
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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