Structure and Function of Trypsin-Loaded Fibrinolytic Liposomes
Joint Authors
Bóta, Attila
Tanka-Salamon, Anna
Wacha, András
Mihály, Judith
Lovas, Miklós
Kolev, Krasimir
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Protease encapsulation and its targeted release in thrombi may contribute to the reduction of haemorrhagic complications of thrombolysis.
We aimed to prepare sterically stabilized trypsin-loaded liposomes (SSLT) and characterize their structure and fibrinolytic efficiency.
Hydrogenated soybean phosphatidylcholine-based SSLT were prepared and their structure was studied by transmission electron microscopy combined with freeze fracture (FF-TEM), Fourier transform infrared spectroscopy (FT-IR), and small-angle X-ray scattering (SAXS).
Fibrinolytic activity was examined at 45, 37, or 24°C on fibrin or plasma clots with turbidimetric and permeation-driven lysis assays.
Trypsin was shown to be attached to the inner surface of vesicles (SAXS and FF-TEM) close to the lipid hydrophilic/hydrophobic interface (FT-IR).
The thermosensitivity of SSLT was evidenced by enhanced fibrinolysis at 45°C: time to reduce the maximal turbidity to 20% decreased by 8.6% compared to 37°C and fibrin degradation product concentration in the permeation lysis assay was 2-fold to 5-fold higher than that at 24°C.
SSLT exerted its fibrinolytic action on fibrin clots under both static and dynamic conditions, whereas plasma clot dissolution was observed only in the permeation-driven assay.
The improved fibrinolytic efficiency of SSLT under dynamic conditions suggests that they may serve as a novel therapeutic candidate for dissolution of intravascular thrombi, which are typically exposed to permeation forces.
American Psychological Association (APA)
Tanka-Salamon, Anna& Bóta, Attila& Wacha, András& Mihály, Judith& Lovas, Miklós& Kolev, Krasimir. 2017. Structure and Function of Trypsin-Loaded Fibrinolytic Liposomes. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1137439
Modern Language Association (MLA)
Tanka-Salamon, Anna…[et al.]. Structure and Function of Trypsin-Loaded Fibrinolytic Liposomes. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1137439
American Medical Association (AMA)
Tanka-Salamon, Anna& Bóta, Attila& Wacha, András& Mihály, Judith& Lovas, Miklós& Kolev, Krasimir. Structure and Function of Trypsin-Loaded Fibrinolytic Liposomes. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1137439
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137439