Pharmacokinetic Study of Thirteen Ingredients after the Oral Administration of Flos Chrysanthemi Extract in Rats by UPLC-MSMS
المؤلفون المشاركون
Chang, Yanxu
He, Jun
Ma, Wenjuan
Yao, Guangzhe
Jia, Qi
Ouyang, Huizi
Huang, Xuhua
Shen, Jiayuan
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-21
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
A rapid and reliable UPLC-MS/MS method was developed and validated for the simultaneous quantification of thirteen bioactive compounds (luteolin, cynaroside, luteolin 7-O-glucuronide, isochlorogenic acid C, chlorogenic acid, cryptochlorogenic acid, apigenin, apigenin 7-glucoside, acacetin, hyperoside, isoquercitrin, tilianin, and hesperidin) in rat plasma.
The compounds were separated on an ACQUITY UPLC BEH C18 column (2.1×100 mm, 1.7 μm) with a gradient mobile phase system of acetonitrile and 0.1% (v/v) formic acid aqueous solution at a flow rate of 0.3 mL/min.
All compounds were quantitated using Agilent Jet Stream electrospray ionization (AJS ESI) in a negative ion mode.
The lower limit of quantification (LLOQ) for all compounds was below 5 ng/mL.
The intra- and interday accuracy ranged from -13.0% to 14.0%, and precisions were less than 12.2%.
The extraction recoveries of the compounds were in the range of 56.9% to 95.0%, and the matrix effect ranged between 71.6% and 109.3%.
Stability studies proved that the thirteen compounds were stable under tested conditions, with a relative standard deviation (RSD) of less than 11.4%.
This developed method was successfully applied to the pharmacokinetic study of the 13 bioactive compounds after oral administration of Flos Chrysanthemi extract in rat by UPLC-MS/MS.
Pharmacokinetic parameters of 8 out of the 13 compounds investigated are presented in this paper.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jia, Qi& Huang, Xuhua& Yao, Guangzhe& Ma, Wenjuan& Shen, Jiayuan& Chang, Yanxu…[et al.]. 2020. Pharmacokinetic Study of Thirteen Ingredients after the Oral Administration of Flos Chrysanthemi Extract in Rats by UPLC-MSMS. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1137452
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jia, Qi…[et al.]. Pharmacokinetic Study of Thirteen Ingredients after the Oral Administration of Flos Chrysanthemi Extract in Rats by UPLC-MSMS. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1137452
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jia, Qi& Huang, Xuhua& Yao, Guangzhe& Ma, Wenjuan& Shen, Jiayuan& Chang, Yanxu…[et al.]. Pharmacokinetic Study of Thirteen Ingredients after the Oral Administration of Flos Chrysanthemi Extract in Rats by UPLC-MSMS. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1137452
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1137452
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر