Pharmacokinetic Study of Thirteen Ingredients after the Oral Administration of Flos Chrysanthemi Extract in Rats by UPLC-MSMS
Joint Authors
Chang, Yanxu
He, Jun
Ma, Wenjuan
Yao, Guangzhe
Jia, Qi
Ouyang, Huizi
Huang, Xuhua
Shen, Jiayuan
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-21
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A rapid and reliable UPLC-MS/MS method was developed and validated for the simultaneous quantification of thirteen bioactive compounds (luteolin, cynaroside, luteolin 7-O-glucuronide, isochlorogenic acid C, chlorogenic acid, cryptochlorogenic acid, apigenin, apigenin 7-glucoside, acacetin, hyperoside, isoquercitrin, tilianin, and hesperidin) in rat plasma.
The compounds were separated on an ACQUITY UPLC BEH C18 column (2.1×100 mm, 1.7 μm) with a gradient mobile phase system of acetonitrile and 0.1% (v/v) formic acid aqueous solution at a flow rate of 0.3 mL/min.
All compounds were quantitated using Agilent Jet Stream electrospray ionization (AJS ESI) in a negative ion mode.
The lower limit of quantification (LLOQ) for all compounds was below 5 ng/mL.
The intra- and interday accuracy ranged from -13.0% to 14.0%, and precisions were less than 12.2%.
The extraction recoveries of the compounds were in the range of 56.9% to 95.0%, and the matrix effect ranged between 71.6% and 109.3%.
Stability studies proved that the thirteen compounds were stable under tested conditions, with a relative standard deviation (RSD) of less than 11.4%.
This developed method was successfully applied to the pharmacokinetic study of the 13 bioactive compounds after oral administration of Flos Chrysanthemi extract in rat by UPLC-MS/MS.
Pharmacokinetic parameters of 8 out of the 13 compounds investigated are presented in this paper.
American Psychological Association (APA)
Jia, Qi& Huang, Xuhua& Yao, Guangzhe& Ma, Wenjuan& Shen, Jiayuan& Chang, Yanxu…[et al.]. 2020. Pharmacokinetic Study of Thirteen Ingredients after the Oral Administration of Flos Chrysanthemi Extract in Rats by UPLC-MSMS. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1137452
Modern Language Association (MLA)
Jia, Qi…[et al.]. Pharmacokinetic Study of Thirteen Ingredients after the Oral Administration of Flos Chrysanthemi Extract in Rats by UPLC-MSMS. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1137452
American Medical Association (AMA)
Jia, Qi& Huang, Xuhua& Yao, Guangzhe& Ma, Wenjuan& Shen, Jiayuan& Chang, Yanxu…[et al.]. Pharmacokinetic Study of Thirteen Ingredients after the Oral Administration of Flos Chrysanthemi Extract in Rats by UPLC-MSMS. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1137452
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137452