![](/images/graphics-bg.png)
Efficient Expression of Maltohexaose-Forming α-Amylase from Bacillus stearothermophilus in Brevibacillus choshinensis SP3 and Its Use in Maltose Production
المؤلفون المشاركون
Li, Zhu
Su, Lingqia
Duan, Xuguo
Wu, Dan
Wu, Jing
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-11-09
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
The maltohexaose-forming, Ca2+-independent α-amylase gene from Bacillus stearothermophilus (AmyMH) was efficiently expressed in Brevibacillus choshinensis SP3.
To improve the production of AmyMH in B.
choshinensis SP3, the temperature and initial pH of culture medium were optimized.
In addition, single-factor and response surface methodologies were pursued to optimize culture medium.
Addition of proline to the culture medium significantly improved the production of recombinant α-amylase in B.
choshinensis SP3.
This improvement may result from improved cellular integrity of recombinant B.
choshinensis SP3 in existence of proline.
Culture medium optimization resulted in an 8-fold improvement in α-amylase yield, which reached 1.72 × 104 U·mL−1.
The recombinant α-amylase was applied to the production of maltose on a laboratory scale.
A maltose content of 90.72%, which could be classified as an extremely high maltose syrup, could be achieved using 15% (m/v) corn starch as the substrate.
This study demonstrated that the B.
choshinensis SP3 expression system was able to produce substantial quantities of recombinant α-amylase that has potential application in the starch industry.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Zhu& Su, Lingqia& Duan, Xuguo& Wu, Dan& Wu, Jing. 2017. Efficient Expression of Maltohexaose-Forming α-Amylase from Bacillus stearothermophilus in Brevibacillus choshinensis SP3 and Its Use in Maltose Production. BioMed Research International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1137656
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Zhu…[et al.]. Efficient Expression of Maltohexaose-Forming α-Amylase from Bacillus stearothermophilus in Brevibacillus choshinensis SP3 and Its Use in Maltose Production. BioMed Research International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1137656
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Zhu& Su, Lingqia& Duan, Xuguo& Wu, Dan& Wu, Jing. Efficient Expression of Maltohexaose-Forming α-Amylase from Bacillus stearothermophilus in Brevibacillus choshinensis SP3 and Its Use in Maltose Production. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1137656
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1137656
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)