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Efficient Expression of Maltohexaose-Forming α-Amylase from Bacillus stearothermophilus in Brevibacillus choshinensis SP3 and Its Use in Maltose Production
Joint Authors
Li, Zhu
Su, Lingqia
Duan, Xuguo
Wu, Dan
Wu, Jing
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-09
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The maltohexaose-forming, Ca2+-independent α-amylase gene from Bacillus stearothermophilus (AmyMH) was efficiently expressed in Brevibacillus choshinensis SP3.
To improve the production of AmyMH in B.
choshinensis SP3, the temperature and initial pH of culture medium were optimized.
In addition, single-factor and response surface methodologies were pursued to optimize culture medium.
Addition of proline to the culture medium significantly improved the production of recombinant α-amylase in B.
choshinensis SP3.
This improvement may result from improved cellular integrity of recombinant B.
choshinensis SP3 in existence of proline.
Culture medium optimization resulted in an 8-fold improvement in α-amylase yield, which reached 1.72 × 104 U·mL−1.
The recombinant α-amylase was applied to the production of maltose on a laboratory scale.
A maltose content of 90.72%, which could be classified as an extremely high maltose syrup, could be achieved using 15% (m/v) corn starch as the substrate.
This study demonstrated that the B.
choshinensis SP3 expression system was able to produce substantial quantities of recombinant α-amylase that has potential application in the starch industry.
American Psychological Association (APA)
Li, Zhu& Su, Lingqia& Duan, Xuguo& Wu, Dan& Wu, Jing. 2017. Efficient Expression of Maltohexaose-Forming α-Amylase from Bacillus stearothermophilus in Brevibacillus choshinensis SP3 and Its Use in Maltose Production. BioMed Research International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1137656
Modern Language Association (MLA)
Li, Zhu…[et al.]. Efficient Expression of Maltohexaose-Forming α-Amylase from Bacillus stearothermophilus in Brevibacillus choshinensis SP3 and Its Use in Maltose Production. BioMed Research International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1137656
American Medical Association (AMA)
Li, Zhu& Su, Lingqia& Duan, Xuguo& Wu, Dan& Wu, Jing. Efficient Expression of Maltohexaose-Forming α-Amylase from Bacillus stearothermophilus in Brevibacillus choshinensis SP3 and Its Use in Maltose Production. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1137656
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137656