Genotyping of Clostridium perfringens Isolates from Domestic Livestock in Saudi Arabia
المؤلفون المشاركون
Omer, Sawsan A.
Al-Olayan, Ebtesam M.
Babiker, Salah Eldin H.
Aljulaifi, Mohammed Z.
Alagaili, Abdulaziz N.
Mohammed, Osama B.
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-03-24
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The present study was undertaken to confirm the genetic identity of Clostridium perfringens isolates from domestic livestock in Saudi Arabia and to characterize the genes encoding to alpha, beta, epsilon, and iota (α-, β-, ε-, and ι-) toxins.
C.
perfringens were confirmed in 104 out of 136 isolates on multiplex polymerase chain reaction using specific primers amplifying genes related to toxins produced by C.
perfringens.
Genes encoding α-toxins were detected in 104 samples.
Of the isolates, 80.8% were diagnosed as type A, 15.4% as type D, 2.9% as type C, and 0.96% as type B.
None of the isolates has genes encoding iota (ι-) toxin.
All isolates investigated yielded enterotoxin (cpe) products and none yielded β2 (cpb2-toxin) or NetB products.
PLC gene sequences encoding α-toxin showed >96.7% similarity.
Isolates which had α-toxins as well as enterotoxin (cpe) are regarded as type F.
Phylogenetic analysis using maximum likelihood analysis yielded two clades, and the majority of the isolates were in one group while only two isolates clustered on the second clade.
Within the Kingdom of Saudi Arabia strains, 54 variable positions and 23 polymorphic amino acids were noticed.
Isolates with ε- and β-toxins were variable and were found to be close to those published for C.
perfringens.
ETX gene sequences encoding ε-toxins were found to be related to CPE sequences.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Omer, Sawsan A.& Al-Olayan, Ebtesam M.& Babiker, Salah Eldin H.& Aljulaifi, Mohammed Z.& Alagaili, Abdulaziz N.& Mohammed, Osama B.. 2020. Genotyping of Clostridium perfringens Isolates from Domestic Livestock in Saudi Arabia. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1137921
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Omer, Sawsan A.…[et al.]. Genotyping of Clostridium perfringens Isolates from Domestic Livestock in Saudi Arabia. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1137921
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Omer, Sawsan A.& Al-Olayan, Ebtesam M.& Babiker, Salah Eldin H.& Aljulaifi, Mohammed Z.& Alagaili, Abdulaziz N.& Mohammed, Osama B.. Genotyping of Clostridium perfringens Isolates from Domestic Livestock in Saudi Arabia. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1137921
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1137921
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر