Genotyping of Clostridium perfringens Isolates from Domestic Livestock in Saudi Arabia
Joint Authors
Omer, Sawsan A.
Al-Olayan, Ebtesam M.
Babiker, Salah Eldin H.
Aljulaifi, Mohammed Z.
Alagaili, Abdulaziz N.
Mohammed, Osama B.
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The present study was undertaken to confirm the genetic identity of Clostridium perfringens isolates from domestic livestock in Saudi Arabia and to characterize the genes encoding to alpha, beta, epsilon, and iota (α-, β-, ε-, and ι-) toxins.
C.
perfringens were confirmed in 104 out of 136 isolates on multiplex polymerase chain reaction using specific primers amplifying genes related to toxins produced by C.
perfringens.
Genes encoding α-toxins were detected in 104 samples.
Of the isolates, 80.8% were diagnosed as type A, 15.4% as type D, 2.9% as type C, and 0.96% as type B.
None of the isolates has genes encoding iota (ι-) toxin.
All isolates investigated yielded enterotoxin (cpe) products and none yielded β2 (cpb2-toxin) or NetB products.
PLC gene sequences encoding α-toxin showed >96.7% similarity.
Isolates which had α-toxins as well as enterotoxin (cpe) are regarded as type F.
Phylogenetic analysis using maximum likelihood analysis yielded two clades, and the majority of the isolates were in one group while only two isolates clustered on the second clade.
Within the Kingdom of Saudi Arabia strains, 54 variable positions and 23 polymorphic amino acids were noticed.
Isolates with ε- and β-toxins were variable and were found to be close to those published for C.
perfringens.
ETX gene sequences encoding ε-toxins were found to be related to CPE sequences.
American Psychological Association (APA)
Omer, Sawsan A.& Al-Olayan, Ebtesam M.& Babiker, Salah Eldin H.& Aljulaifi, Mohammed Z.& Alagaili, Abdulaziz N.& Mohammed, Osama B.. 2020. Genotyping of Clostridium perfringens Isolates from Domestic Livestock in Saudi Arabia. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1137921
Modern Language Association (MLA)
Omer, Sawsan A.…[et al.]. Genotyping of Clostridium perfringens Isolates from Domestic Livestock in Saudi Arabia. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1137921
American Medical Association (AMA)
Omer, Sawsan A.& Al-Olayan, Ebtesam M.& Babiker, Salah Eldin H.& Aljulaifi, Mohammed Z.& Alagaili, Abdulaziz N.& Mohammed, Osama B.. Genotyping of Clostridium perfringens Isolates from Domestic Livestock in Saudi Arabia. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1137921
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137921