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Pharmacokinetic Effects of l-Tetrahydropalmatine on Ketamine in Rat Plasma by Ultraperformance Liquid Chromatography Tandem Mass Spectrometry
المؤلفون المشاركون
Du, Yan
Su, Hongliang
Cao, Jie
Wei, Zhiwen
Wang, Yujin
Yun, Keming
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-07-07
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Male Sprague-Dawley rats (n=18) were randomly divided into three groups: a saline group (20 mL/kg by gavage), a ketamine (KET) group (100 mg/kg by gavage), and a KET (the same routes and doses) combined with levo-tetrahydropalmatine (l-THP; 40 mg/kg by gavage) group (n=6).
Blood samples were acquired at different time points after drug administration.
A simple and sensitive ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was established to determine the concentrations of KET and its metabolite, norketamine (NK), in rat plasma.
Chromatographic separation was achieved using a BEH C18 column (2.1 mm×50 mm, 1.7 μm) with chlorpheniramine maleate (Chlor-Trimeton) as an internal standard (IS).
The initial mobile phase consisted of acetonitrile–water with 0.1% methanoic acid (80 : 20, v/v).
The multiple reaction monitoring (MRM) modes of m/z 238.1→m/z 179.1 for KET, m/z 224.1→m/z 207.1 for NK, and m/z 275→m/z 230 for Chlor-Trimeton (IS) were utilized to conduct a quantitative analysis.
Calibration curves of KET and NK in rat plasma demonstrated good linearity in the range of 2.5–500 ng/mL (r>0.9994), and the lower limit of quantification (LLOQ) was 2.5 ng/mL for both.
Moreover, the intra- and interday precision relative standard deviation (RSD) of KET and NK were less than 4.31% and 6.53%, respectively.
The accuracies (relative error) of KET and NK were below -1.41% and -6.07%, respectively.
The extraction recoveries of KET and NK were more than 81.23±3.45% and 80.42±4.57%, respectively.
This sensitive, rapid, and selective UPLC-MS/MS method was successfully applied to study the pharmacokinetic effects of l-THP on KET after gastric gavage.
The results demonstrated that l-THP could increase the bioavailability of KET and promote the metabolism of KET.
The results showed that l-THP has pharmacokinetics effects on KET in rat plasma.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Du, Yan& Su, Hongliang& Cao, Jie& Wei, Zhiwen& Wang, Yujin& Yun, Keming. 2020. Pharmacokinetic Effects of l-Tetrahydropalmatine on Ketamine in Rat Plasma by Ultraperformance Liquid Chromatography Tandem Mass Spectrometry. BioMed Research International،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1138039
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Du, Yan…[et al.]. Pharmacokinetic Effects of l-Tetrahydropalmatine on Ketamine in Rat Plasma by Ultraperformance Liquid Chromatography Tandem Mass Spectrometry. BioMed Research International No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1138039
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Du, Yan& Su, Hongliang& Cao, Jie& Wei, Zhiwen& Wang, Yujin& Yun, Keming. Pharmacokinetic Effects of l-Tetrahydropalmatine on Ketamine in Rat Plasma by Ultraperformance Liquid Chromatography Tandem Mass Spectrometry. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1138039
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1138039
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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