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Pharmacokinetic Effects of l-Tetrahydropalmatine on Ketamine in Rat Plasma by Ultraperformance Liquid Chromatography Tandem Mass Spectrometry
Joint Authors
Du, Yan
Su, Hongliang
Cao, Jie
Wei, Zhiwen
Wang, Yujin
Yun, Keming
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-07
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Male Sprague-Dawley rats (n=18) were randomly divided into three groups: a saline group (20 mL/kg by gavage), a ketamine (KET) group (100 mg/kg by gavage), and a KET (the same routes and doses) combined with levo-tetrahydropalmatine (l-THP; 40 mg/kg by gavage) group (n=6).
Blood samples were acquired at different time points after drug administration.
A simple and sensitive ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was established to determine the concentrations of KET and its metabolite, norketamine (NK), in rat plasma.
Chromatographic separation was achieved using a BEH C18 column (2.1 mm×50 mm, 1.7 μm) with chlorpheniramine maleate (Chlor-Trimeton) as an internal standard (IS).
The initial mobile phase consisted of acetonitrile–water with 0.1% methanoic acid (80 : 20, v/v).
The multiple reaction monitoring (MRM) modes of m/z 238.1→m/z 179.1 for KET, m/z 224.1→m/z 207.1 for NK, and m/z 275→m/z 230 for Chlor-Trimeton (IS) were utilized to conduct a quantitative analysis.
Calibration curves of KET and NK in rat plasma demonstrated good linearity in the range of 2.5–500 ng/mL (r>0.9994), and the lower limit of quantification (LLOQ) was 2.5 ng/mL for both.
Moreover, the intra- and interday precision relative standard deviation (RSD) of KET and NK were less than 4.31% and 6.53%, respectively.
The accuracies (relative error) of KET and NK were below -1.41% and -6.07%, respectively.
The extraction recoveries of KET and NK were more than 81.23±3.45% and 80.42±4.57%, respectively.
This sensitive, rapid, and selective UPLC-MS/MS method was successfully applied to study the pharmacokinetic effects of l-THP on KET after gastric gavage.
The results demonstrated that l-THP could increase the bioavailability of KET and promote the metabolism of KET.
The results showed that l-THP has pharmacokinetics effects on KET in rat plasma.
American Psychological Association (APA)
Du, Yan& Su, Hongliang& Cao, Jie& Wei, Zhiwen& Wang, Yujin& Yun, Keming. 2020. Pharmacokinetic Effects of l-Tetrahydropalmatine on Ketamine in Rat Plasma by Ultraperformance Liquid Chromatography Tandem Mass Spectrometry. BioMed Research International،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1138039
Modern Language Association (MLA)
Du, Yan…[et al.]. Pharmacokinetic Effects of l-Tetrahydropalmatine on Ketamine in Rat Plasma by Ultraperformance Liquid Chromatography Tandem Mass Spectrometry. BioMed Research International No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1138039
American Medical Association (AMA)
Du, Yan& Su, Hongliang& Cao, Jie& Wei, Zhiwen& Wang, Yujin& Yun, Keming. Pharmacokinetic Effects of l-Tetrahydropalmatine on Ketamine in Rat Plasma by Ultraperformance Liquid Chromatography Tandem Mass Spectrometry. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1138039
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138039