Optimization, Purification, and Starch Stain Wash Application of Two New α-Amylases Extracted from Leaves and Stems of Pergularia tomentosa
المؤلفون المشاركون
El Abed, Hanen
Khemakhem, Bassem
Belghith, K. S.
Lahmar, Imen
Belghith, Hafedh
Ben Abdallah, Ferjani
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-12-17
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
A continuous research is attempted to fulfil the highest industrial demands of natural amylases presenting special properties.
New α-amylases extracted from stems and leaves of Pergularia tomentosa, which is widespread and growing spontaneously in Tunisia, were studied by the means of their activities optimization and purification.
Some similarities were recorded for the two identified enzymes: (i) the highest amylase activity showed a promoted thermal stability at 50°C; (ii) the starch substrate at 1% enhanced the enzyme activity; (iii) the two α-amylases seem to be calcium-independent; (iv) Zn2+, Cu2+, and Ag2+ were considered as important inhibitors of the enzyme activity.
Following the increased gradient of elution on Mono Q-Sepharose column, an increase in the specific activity of 11.82-fold and 10.92-fold was recorded, respectively, for leaves and stems with the presence of different peaks on the purification profiles.
Pergularia amylases activities were stable and compatible with the tested commercial detergents.
The combination of plant amylase and detergent allowed us to enhance the wash performance with an increase of 35.24 and 42.56%, respectively, for stems and leaves amylases.
Characterized amylases were reported to have a promoted potential for their implication notably in detergent industry as well as biotechnological sector.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lahmar, Imen& El Abed, Hanen& Khemakhem, Bassem& Belghith, Hafedh& Ben Abdallah, Ferjani& Belghith, K. S.. 2017. Optimization, Purification, and Starch Stain Wash Application of Two New α-Amylases Extracted from Leaves and Stems of Pergularia tomentosa. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1138180
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lahmar, Imen…[et al.]. Optimization, Purification, and Starch Stain Wash Application of Two New α-Amylases Extracted from Leaves and Stems of Pergularia tomentosa. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1138180
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lahmar, Imen& El Abed, Hanen& Khemakhem, Bassem& Belghith, Hafedh& Ben Abdallah, Ferjani& Belghith, K. S.. Optimization, Purification, and Starch Stain Wash Application of Two New α-Amylases Extracted from Leaves and Stems of Pergularia tomentosa. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1138180
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1138180
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر