Optimization, Purification, and Starch Stain Wash Application of Two New α-Amylases Extracted from Leaves and Stems of Pergularia tomentosa
Joint Authors
El Abed, Hanen
Khemakhem, Bassem
Belghith, K. S.
Lahmar, Imen
Belghith, Hafedh
Ben Abdallah, Ferjani
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
A continuous research is attempted to fulfil the highest industrial demands of natural amylases presenting special properties.
New α-amylases extracted from stems and leaves of Pergularia tomentosa, which is widespread and growing spontaneously in Tunisia, were studied by the means of their activities optimization and purification.
Some similarities were recorded for the two identified enzymes: (i) the highest amylase activity showed a promoted thermal stability at 50°C; (ii) the starch substrate at 1% enhanced the enzyme activity; (iii) the two α-amylases seem to be calcium-independent; (iv) Zn2+, Cu2+, and Ag2+ were considered as important inhibitors of the enzyme activity.
Following the increased gradient of elution on Mono Q-Sepharose column, an increase in the specific activity of 11.82-fold and 10.92-fold was recorded, respectively, for leaves and stems with the presence of different peaks on the purification profiles.
Pergularia amylases activities were stable and compatible with the tested commercial detergents.
The combination of plant amylase and detergent allowed us to enhance the wash performance with an increase of 35.24 and 42.56%, respectively, for stems and leaves amylases.
Characterized amylases were reported to have a promoted potential for their implication notably in detergent industry as well as biotechnological sector.
American Psychological Association (APA)
Lahmar, Imen& El Abed, Hanen& Khemakhem, Bassem& Belghith, Hafedh& Ben Abdallah, Ferjani& Belghith, K. S.. 2017. Optimization, Purification, and Starch Stain Wash Application of Two New α-Amylases Extracted from Leaves and Stems of Pergularia tomentosa. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1138180
Modern Language Association (MLA)
Lahmar, Imen…[et al.]. Optimization, Purification, and Starch Stain Wash Application of Two New α-Amylases Extracted from Leaves and Stems of Pergularia tomentosa. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1138180
American Medical Association (AMA)
Lahmar, Imen& El Abed, Hanen& Khemakhem, Bassem& Belghith, Hafedh& Ben Abdallah, Ferjani& Belghith, K. S.. Optimization, Purification, and Starch Stain Wash Application of Two New α-Amylases Extracted from Leaves and Stems of Pergularia tomentosa. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1138180
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138180