Isolation and Abiotic Stress Resistance Analyses of a Catalase Gene from Ipomoea batatas (L.)‎ Lam

المؤلفون المشاركون

Yong, Bin
Wang, Xiaoyan
Xu, Pan
Zheng, Haiyan
Fei, Xueting
Hong, Zixi
Ma, Qinqin
Miao, Yuzhi
Yuan, Xianghua
Jiang, Yusong
Shao, Huanhuan

المصدر

BioMed Research International

العدد

المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2017-05-30

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الطب البشري

الملخص EN

As an indicator of the antioxidant capability of plants, catalase can detoxify reactive oxygen species (ROS) generated by environmental stresses.

Sweet potato is one of the top six most important crops in the world.

However, its catalases remain largely unknown.

In this study, a catalase encoding gene, IbCAT2 (accession number: KY615708), was identified and cloned from sweet potato cv.

Xushu 18.

It contained a 1479 nucleotides’ open reading frame (ORF).

S-R-L, Q-K-L, and a putative calmodulin binding domain were located at the C-terminus of IbCAT2, which suggests that IbCAT2 could be a peroxisomal catalase.

Next-generation sequencing (NGS) based quantitative analyses showed that IbCAT2 was mainly expressed in young leaves and expanding tuberous roots under normal conditions.

When exposed to 10% PEG6000 or 200 mmol/L NaCl solutions, IbCAT2 was upregulated rapidly in the first 11 days and then downregulated, although different tissues showed different degree of change.

Overexpression of IbCAT2 conferred salt and drought tolerance in Escherichia coli and Saccharomyces cerevisiae.

The positive response of IbCAT2 to abiotic stresses suggested that IbCAT2 might play an important role in stress responses.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Yong, Bin& Wang, Xiaoyan& Xu, Pan& Zheng, Haiyan& Fei, Xueting& Hong, Zixi…[et al.]. 2017. Isolation and Abiotic Stress Resistance Analyses of a Catalase Gene from Ipomoea batatas (L.) Lam. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1138245

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Yong, Bin…[et al.]. Isolation and Abiotic Stress Resistance Analyses of a Catalase Gene from Ipomoea batatas (L.) Lam. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1138245

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Yong, Bin& Wang, Xiaoyan& Xu, Pan& Zheng, Haiyan& Fei, Xueting& Hong, Zixi…[et al.]. Isolation and Abiotic Stress Resistance Analyses of a Catalase Gene from Ipomoea batatas (L.) Lam. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1138245

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1138245