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Isolation and Abiotic Stress Resistance Analyses of a Catalase Gene from Ipomoea batatas (L.) Lam
Joint Authors
Yong, Bin
Wang, Xiaoyan
Xu, Pan
Zheng, Haiyan
Fei, Xueting
Hong, Zixi
Ma, Qinqin
Miao, Yuzhi
Yuan, Xianghua
Jiang, Yusong
Shao, Huanhuan
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
As an indicator of the antioxidant capability of plants, catalase can detoxify reactive oxygen species (ROS) generated by environmental stresses.
Sweet potato is one of the top six most important crops in the world.
However, its catalases remain largely unknown.
In this study, a catalase encoding gene, IbCAT2 (accession number: KY615708), was identified and cloned from sweet potato cv.
Xushu 18.
It contained a 1479 nucleotides’ open reading frame (ORF).
S-R-L, Q-K-L, and a putative calmodulin binding domain were located at the C-terminus of IbCAT2, which suggests that IbCAT2 could be a peroxisomal catalase.
Next-generation sequencing (NGS) based quantitative analyses showed that IbCAT2 was mainly expressed in young leaves and expanding tuberous roots under normal conditions.
When exposed to 10% PEG6000 or 200 mmol/L NaCl solutions, IbCAT2 was upregulated rapidly in the first 11 days and then downregulated, although different tissues showed different degree of change.
Overexpression of IbCAT2 conferred salt and drought tolerance in Escherichia coli and Saccharomyces cerevisiae.
The positive response of IbCAT2 to abiotic stresses suggested that IbCAT2 might play an important role in stress responses.
American Psychological Association (APA)
Yong, Bin& Wang, Xiaoyan& Xu, Pan& Zheng, Haiyan& Fei, Xueting& Hong, Zixi…[et al.]. 2017. Isolation and Abiotic Stress Resistance Analyses of a Catalase Gene from Ipomoea batatas (L.) Lam. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1138245
Modern Language Association (MLA)
Yong, Bin…[et al.]. Isolation and Abiotic Stress Resistance Analyses of a Catalase Gene from Ipomoea batatas (L.) Lam. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1138245
American Medical Association (AMA)
Yong, Bin& Wang, Xiaoyan& Xu, Pan& Zheng, Haiyan& Fei, Xueting& Hong, Zixi…[et al.]. Isolation and Abiotic Stress Resistance Analyses of a Catalase Gene from Ipomoea batatas (L.) Lam. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1138245
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138245