Extracellular Signal-Regulated Kinase 5 is Required for Low-Concentration H2O2-Induced Angiogenesis of Human Umbilical Vein Endothelial Cells
المؤلفون المشاركون
Han, Wei-Dong
Han, Yan
Huang, Hong
Jiang, Shan
Zhang, Dongxin
Lei, Yonghong
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-04-30
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Background.
The aim of this study was to assess the effects of low concentrations of H2O2 on angiogenesis of human umbilical vein endothelial cells (HUVECs) in vitro and explore the underlying mechanisms.
Methods.
HUVECs were cultured and stimulated with different concentrations of H2O2.
Flow cytometric analysis was used to select an optimal concentration of H2O2 for the following experiments.
Cell proliferation, migration, and tubule formation were evaluated by Cell Counting Kit-8 (CCK-8) assays, scratch wound assays, and Matrigel tubule formation assays, respectively.
For gain and loss of function studies, constitutively active MEK5 (CA-MEK5) and ERK5 shRNA lentiviruses were used to activate or knock down extracellular signal-regulated kinase 5 (ERK5).
Results.
We found that low concentrations of H2O2 promoted HUVECs proliferation, migration, and tubule formation.
ERK5 in HUVECs was significantly activated by H2O2.
Enhanced ERK5 activity significantly amplified the proangiogenic effects of H2O2; in contrast, ERK5 knock-down abrogated the effects of H2O2.
Conclusions.
Our results confirmed that low concentrations of H2O2 promoted HUVECs angiogenesis in vitro, and ERK5 is an essential mediator of this process.
Therefore, ERK5 may be a potential therapeutic target for promoting angiogenesis and improving graft survival.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jiang, Shan& Zhang, Dongxin& Huang, Hong& Lei, Yonghong& Han, Yan& Han, Wei-Dong. 2017. Extracellular Signal-Regulated Kinase 5 is Required for Low-Concentration H2O2-Induced Angiogenesis of Human Umbilical Vein Endothelial Cells. BioMed Research International،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1138271
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jiang, Shan…[et al.]. Extracellular Signal-Regulated Kinase 5 is Required for Low-Concentration H2O2-Induced Angiogenesis of Human Umbilical Vein Endothelial Cells. BioMed Research International No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1138271
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jiang, Shan& Zhang, Dongxin& Huang, Hong& Lei, Yonghong& Han, Yan& Han, Wei-Dong. Extracellular Signal-Regulated Kinase 5 is Required for Low-Concentration H2O2-Induced Angiogenesis of Human Umbilical Vein Endothelial Cells. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1138271
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1138271
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر