Extracellular Signal-Regulated Kinase 5 is Required for Low-Concentration H2O2-Induced Angiogenesis of Human Umbilical Vein Endothelial Cells
Joint Authors
Han, Wei-Dong
Han, Yan
Huang, Hong
Jiang, Shan
Zhang, Dongxin
Lei, Yonghong
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-30
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Background.
The aim of this study was to assess the effects of low concentrations of H2O2 on angiogenesis of human umbilical vein endothelial cells (HUVECs) in vitro and explore the underlying mechanisms.
Methods.
HUVECs were cultured and stimulated with different concentrations of H2O2.
Flow cytometric analysis was used to select an optimal concentration of H2O2 for the following experiments.
Cell proliferation, migration, and tubule formation were evaluated by Cell Counting Kit-8 (CCK-8) assays, scratch wound assays, and Matrigel tubule formation assays, respectively.
For gain and loss of function studies, constitutively active MEK5 (CA-MEK5) and ERK5 shRNA lentiviruses were used to activate or knock down extracellular signal-regulated kinase 5 (ERK5).
Results.
We found that low concentrations of H2O2 promoted HUVECs proliferation, migration, and tubule formation.
ERK5 in HUVECs was significantly activated by H2O2.
Enhanced ERK5 activity significantly amplified the proangiogenic effects of H2O2; in contrast, ERK5 knock-down abrogated the effects of H2O2.
Conclusions.
Our results confirmed that low concentrations of H2O2 promoted HUVECs angiogenesis in vitro, and ERK5 is an essential mediator of this process.
Therefore, ERK5 may be a potential therapeutic target for promoting angiogenesis and improving graft survival.
American Psychological Association (APA)
Jiang, Shan& Zhang, Dongxin& Huang, Hong& Lei, Yonghong& Han, Yan& Han, Wei-Dong. 2017. Extracellular Signal-Regulated Kinase 5 is Required for Low-Concentration H2O2-Induced Angiogenesis of Human Umbilical Vein Endothelial Cells. BioMed Research International،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1138271
Modern Language Association (MLA)
Jiang, Shan…[et al.]. Extracellular Signal-Regulated Kinase 5 is Required for Low-Concentration H2O2-Induced Angiogenesis of Human Umbilical Vein Endothelial Cells. BioMed Research International No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1138271
American Medical Association (AMA)
Jiang, Shan& Zhang, Dongxin& Huang, Hong& Lei, Yonghong& Han, Yan& Han, Wei-Dong. Extracellular Signal-Regulated Kinase 5 is Required for Low-Concentration H2O2-Induced Angiogenesis of Human Umbilical Vein Endothelial Cells. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1138271
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138271