A Novel Capillary Electrophoresis-Based High-Throughput Multiplex Polymerase Chain Reaction System for the Simultaneous Detection of Nine Pathogens in Swine
المؤلفون المشاركون
Wu, Xu-long
Xiao, Lu
Lin, Hua
Yang, Miao
Chen, Shi-jie
An, Wei
Wang, Yin
Yao, Xue-ping
Yang, Ze-xiao
Tang, Zi-zhong
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-06-11
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Here we aimed to develop a capillary electrophoresis-based high-throughput multiplex polymerase chain reaction (PCR) system for the simultaneous detection of nine pathogens in swine.
Nine pairs of specific primers and a set of universal primers were designed; the multiplex PCR was established.
The specificity and cross-reactivity of this assay were examined, and the detection limit was determined using serial 10-fold dilutions of plasmids containing the target sequences.
The assay was further tested using 144 clinical samples.
We found that the nine specific amplification peaks were observed, and the assay had a high degree of specificity, without nonspecific amplification.
The simultaneous detection limit for the nine viruses reached 10000 copies μL−1 when all of the premixed viral targets were present.
Seventy-seven of the clinical samples tested positive for at least one of the viruses; the principal viral infections in the clinical samples were porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus.
This approach has much potential for further development of high-throughput detection tools for the diagnosis of diseases in animals.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wu, Xu-long& Xiao, Lu& Lin, Hua& Yang, Miao& Chen, Shi-jie& An, Wei…[et al.]. 2017. A Novel Capillary Electrophoresis-Based High-Throughput Multiplex Polymerase Chain Reaction System for the Simultaneous Detection of Nine Pathogens in Swine. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1138471
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wu, Xu-long…[et al.]. A Novel Capillary Electrophoresis-Based High-Throughput Multiplex Polymerase Chain Reaction System for the Simultaneous Detection of Nine Pathogens in Swine. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1138471
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wu, Xu-long& Xiao, Lu& Lin, Hua& Yang, Miao& Chen, Shi-jie& An, Wei…[et al.]. A Novel Capillary Electrophoresis-Based High-Throughput Multiplex Polymerase Chain Reaction System for the Simultaneous Detection of Nine Pathogens in Swine. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1138471
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1138471
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر